Ye J H, Goo Y S
Department of Physiology, Chungbuk National University School of Medicine, Cheongju, 361-763, Korea.
Physiol Meas. 2007 Sep;28(9):1079-88. doi: 10.1088/0967-3334/28/9/009. Epub 2007 Sep 5.
We investigated the differences in the retinal activity between normal and degenerate retina. Multi-electrode recordings were performed in in vitro mice retinas. Only short duration (<2 ms) retinal spikes were recorded in normal mice by postnatal day 28. However, in rd/rd mice, a slow wave component with approximately 100 ms duration was also recorded along with the spikes. We attempted to understand the mechanism of this slow wave component in degenerate retina by applying various synaptic blockers. With CNQX/AP-7, the glutamate antagonist (n = 7), the slow wave component disappeared while the normally less-dominant retinal spikes became more apparent. With strychnine, the glycine antagonist (n = 3) or picrotoxin, GABA antagonist (n = 3), the amplitude of the slow wave component increased. These suggest that a stronger excitatory glutamate input from bipolar cells to ganglion cells is the main contributor to this slow wave component in rd/rd mice.
我们研究了正常视网膜与退化视网膜之间视网膜活动的差异。在体外小鼠视网膜上进行了多电极记录。到出生后第28天，在正常小鼠中仅记录到持续时间较短（<2毫秒）的视网膜尖峰。然而，在rd/rd小鼠中，除了尖峰外，还记录到了持续时间约为100毫秒的慢波成分。我们试图通过应用各种突触阻滞剂来了解退化视网膜中这种慢波成分的机制。使用谷氨酸拮抗剂CNQX/AP-7（n = 7）时，慢波成分消失，而正常情况下不太占主导的视网膜尖峰变得更加明显。使用甘氨酸拮抗剂士的宁（n = 3）或GABA拮抗剂印防己毒素（n = 3）时，慢波成分的振幅增加。这些表明，来自双极细胞到神经节细胞的更强兴奋性谷氨酸输入是rd/rd小鼠中这种慢波成分的主要贡献因素。
