Popov A V, Ketlinskiĭ S A, Tararak E M
Arkh Patol. 1975;37(10):25-30.
Transport of serous pre-beta and beta-lipopropeins (beta-LP) labeled by C14 protein and by C14 lipid into the wall of the aorta was studied histoautoradiographically and biochemically. The aortas of healthy rabbits and rabbits wuth experimental atherosclerosis were perfused in vitro with a medium containing rabbit pre-beta and beta-LP during 6 hours. After perfusion the mark was identified histoautoradiographically in all layers of the aorta. The presence of gradient in distribution of the mark from the inner layers of the aortas towards the outward part of the media indicated that the process of infiltration of the wall of the LP vessel was caused by their penetration from the lumen of the aorta. Nonuniformity in distribution of LP penetrated into the atherosclerotic aorta was noted, which was considered in connection with the changes occurred in the connective tissue of the wall of the aorta in the process of atherogenesis. Results of both morphological and biochemical investigations confirm an increased permeability of the aorta wall for LP in atherosclerosis and also substantiate the assumption about penetration of pre-beta and beta-LP into the wall of the vessel as a whole particle.
采用组织放射自显影术和生物化学方法，研究了用C14蛋白和C14脂质标记的浆液前β脂蛋白和β脂蛋白（β-LP）向主动脉壁的转运。将健康兔和实验性动脉粥样硬化兔的主动脉在体外灌注含兔前β脂蛋白和β脂蛋白的培养基6小时。灌注后，通过组织放射自显影术在主动脉的所有层中鉴定标记物。标记物从主动脉内层向中膜外侧部分分布存在梯度，这表明LP血管壁的浸润过程是由它们从主动脉腔的渗透引起的。注意到渗透到动脉粥样硬化主动脉中的LP分布不均匀，这与动脉粥样硬化形成过程中主动脉壁结缔组织发生的变化有关。形态学和生物化学研究结果均证实，在动脉粥样硬化中主动脉壁对LP的通透性增加，也证实了前β脂蛋白和β脂蛋白作为完整颗粒渗透到血管壁的假设。
