Nishida T, Nakamura M, Mishima H, Otori T
Department of Ophthalmology, Kinki University School of Medicine, Osaka, Japan.
Exp Eye Res. 1991 Dec;53(6):753-8. doi: 10.1016/0014-4835(91)90110-z.
Hyaluronan (hyaluronic acid), well-known for its viscoelastic properties, is also recognized as a biological signal to cells. Using organ cultures of the rabbit cornea, we investigated the effects of hyaluronan on the migration of corneal epithelium. The addition of hyaluronan to the culture medium increased the length of the path of the corneal epithelial layer in a dose-dependent fashion. Other glycosaminoglycans (chondroitin, chondroitin sulphate, keratan sulphate and heparan sulphate) were also tried, but only hyaluronan exhibited a stimulatory effect on corneal epithelial migration. The effects of hyaluronan and fibronectin or epidermal growth factor (EGF) were additive; the addition of antisera against fibronectin or against EGF did not alter the stimulatory effect of hyaluronan. These results demonstrate that hyaluronan stimulates corneal epithelial migration by mechanism(s) different from those of fibronectin and EGF.
透明质酸以其粘弹性特性而闻名，它也被认为是一种对细胞的生物信号。我们使用兔角膜器官培养物，研究了透明质酸对角膜上皮细胞迁移的影响。向培养基中添加透明质酸以剂量依赖的方式增加了角膜上皮层的迁移路径长度。我们还尝试了其他糖胺聚糖（软骨素、硫酸软骨素、硫酸角质素和硫酸乙酰肝素），但只有透明质酸对角膜上皮细胞迁移表现出刺激作用。透明质酸与纤连蛋白或表皮生长因子（EGF）的作用是相加的；添加抗纤连蛋白或抗EGF的抗血清并没有改变透明质酸的刺激作用。这些结果表明，透明质酸通过与纤连蛋白和EGF不同的机制刺激角膜上皮细胞迁移。
