Science. 1962 Nov 2;138(3540):594-5. doi: 10.1126/science.138.3540.594.
The zona pellucida may be removed from all stages of the mouse egg by digestion with pronase. Cumulus and corona cells are also dispersed by the enzyme. No change in membrane disgestibility occurs at fertilization. In tests thus far of two-cell eggs and later stages, development continues. Blastocysts exhibit "hatching" behavior despite absence of the zona. Functions of the zona include maintenance of the normal cleavage pattern and prevention of egg fusion.
透明带可以通过蛋白酶消化从老鼠卵子的所有阶段中去除。卵丘和冠状细胞也被该酶分散。在受精时，膜的消化性没有变化。在迄今为止的两个细胞期卵子和后期阶段的测试中，发育继续进行。尽管没有透明带，胚泡仍表现出“孵化”行为。透明带的功能包括维持正常的卵裂模式和防止卵子融合。
Zotero 插件