Yeast artificial chromosome-based genome mapping: some lessons from Xq24-q28.

作者信息

Schlessinger D, Little R D, Freije D, Abidi F, Zucchi I, Porta G, Pilia G, Nagaraja R, Johnson S K, Yoon J Y, Srivastava A, Kere J, Palmieri G, Ciccodicola A, Montanaro V, Romano G, Casamassimi A, D'Urso M

机构信息

Department of Molecular Microbiology, Washington University School Medicine, St. Louis, Missouri 63110.

出版信息

Genomics. 1991 Dec;11(4):783-93. doi: 10.1016/0888-7543(91)90001-u.

Yeast artificial chromosomes (YACs) have recently provided a potential route to long-range coverage of complex genomes in contiguous cloned DNA. In a pilot project for 50 Mb (1.5% of the human genome), a variety of techniques have been applied to assemble Xq24-q28 YAC contigs up to 8 Mb in length and assess their quality. The results indicate the relative strength of several approaches and support the adequacy of YAC-based methods for mapping the human genome.