Science. 1946 Jun 28;103(2687):758-9. doi: 10.1126/science.103.2687.758.
Starch tryptone agar containing 1 : 20,000 Tergitol-7 was successfully sterilized in the Arnold by one 30-minute heating period. Decreasing the concentration to 1 : 30,000 resulted in 4 per cent failures. A concentration of 1 : 20,000 in the cold killed spores of B. mycoides and an unidentified heat-resistant bacillus but failed to kill spores of B. subtilis. Concentrations of 1 : 30,000 permitted growth after sterilization of all species of Streptomyces which were tested, while concentrations of 1 : 20,000 permitted growth of all species but one. Concentrations of 1 : 20,000 did not inhibit the growth of selected species of Penicillium and Aspergillus or of Staph. aureus. Concentrations as high as 1 : 10,000 did not inhibit the growth of E. coli.
淀粉胰蛋白胨琼脂中含有 1:20000 的特里戈尔-7，在阿诺德（压力蒸汽灭菌器）中经过一次 30 分钟的加热周期可成功灭菌。将浓度降低至 1:30000 会导致 4%的失败率。浓度为 1:20000 的冷杀菌可杀死蕈状芽孢杆菌和一种未鉴定的耐热杆菌的孢子，但不能杀死枯草芽孢杆菌的孢子。1:30000 的浓度允许所有测试的链霉菌种在灭菌后生长，而 1:20000 的浓度允许除一种以外的所有种生长。1:20000 的浓度不会抑制所选的青霉和曲霉属以及金黄色葡萄球菌的生长。高达 1:10000 的浓度不会抑制大肠杆菌的生长。
