Casella M L, Schmidt-Lorenz W
Department of Food Science, Swiss Federal Institute of Technology (ETH), Zürich.
Zentralbl Hyg Umweltmed. 1989 Oct;189(1):37-49.
The influence of 0.1% peptone, 0.2% albumin, 1% mucin solutions and whole human blood on the inactivation of Staphylococcus aureus ATCC 6538 and spores of Bacillus subtilis var. niger DSM 675 was determined. The bactericidal and sporicidal effectiveness of 0.75, 1.5 and 3.2 mg HCHO l-1 air at temperatures of 35, 40, 45 degrees C and a relative humidity (RH) of 90% decreased in the following order of loading substances: peptone, albumin, mucin and blood. The calculated D-values of the microorganisms suspended and dried in 0.1% petone and in 0.2% albumin after exposure to 3.2 mg HCHO l-1 air at 40 degrees C and a relative humidity of approximately 90% were in both suspensions 1.9 min for S. aureus and 6.1 and 7.3 min respectively for B. subtilis spores. Under the same exposure conditions but with an addition of 1% mucin D-values of 2.5 min and 7.7 min for S. aureus and B. subtilis spores respectively were found. In the presence of blood, D-values of 12.3 and 18.3 min were obtained under the same conditions for S. aureus and B. subtilis spores respectively. Thus the suspension in blood caused a 2-fold increase in D-value compared to the other substances. The nature of the anticoagulant in the whole blood did not cause much difference in the inactivation of B.subtilis spores. Decreasing concentrations of blood caused an increase in sensitivity of B.subtilis spores to gaseous formaldehyde, whereby diluting blood 1:6 reduced the D-value from 23.9 min to only 7.1 min.
测定了0.1%蛋白胨、0.2%白蛋白、1%粘蛋白溶液和全血对金黄色葡萄球菌ATCC 6538以及枯草芽孢杆菌黑色变种DSM 675孢子灭活的影响。在35、40、45℃温度和90%相对湿度（RH）条件下，0.75、1.5和3.2 mg HCHO l-1空气的杀菌和杀孢子效果按照负载物质的以下顺序降低：蛋白胨、白蛋白、粘蛋白和血液。在40℃和大约90%相对湿度下，将微生物悬浮并干燥在0.1%蛋白胨和0.2%白蛋白中，暴露于3.2 mg HCHO l-1空气后，两种悬浮液中金黄色葡萄球菌的D值均为1.9分钟，枯草芽孢杆菌孢子的D值分别为6.1分钟和7.3分钟。在相同暴露条件下，但添加1%粘蛋白时，金黄色葡萄球菌和枯草芽孢杆菌孢子的D值分别为2.5分钟和7.7分钟。在有血液存在的情况下，在相同条件下金黄色葡萄球菌和枯草芽孢杆菌孢子的D值分别为12.3分钟和18.3分钟。因此，与其他物质相比，血液中的悬浮液使D值增加了2倍。全血中抗凝剂的性质对枯草芽孢杆菌孢子的灭活没有太大差异。血液浓度降低导致枯草芽孢杆菌孢子对气态甲醛的敏感性增加，将血液稀释1:6可使D值从23.9分钟降至仅7.1分钟。
