Wilcox M H, Williams P, Smith D G, Modun B, Finch R G, Denyer S P
Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
J Gen Microbiol. 1991 Nov;137(11):2561-70. doi: 10.1099/00221287-137-11-2561.
Strains of coagulase-negative staphylococci (CNS), including Staphylococcus epidermidis, S. hominis, S. warnerii, S. simulans, S. capitis, S. haemolyticus and S. saprophyticus, were isolated from patients with continuous-ambulatory-peritoneal-dialysis-related peritonitis. The cell wall and cytoplasmic membrane protein profiles of CNS strains cultured in either nutrient broth (NB) or pooled human peritoneal dialysate (HPD) were compared. Some interspecies variation in both the wall and membrane protein profiles was noted. However, the cell wall protein profiles of HPD-grown CNS strains differed markedly from those cultured in NB. Growth in HPD resulted in a marked reduction in the total number of cell-wall-associated proteins but up to three antigenically related proteins in the 40-56 kDa range, depending on the species, predominated. Growth in HPD also resulted in the induction of two iron-repressible cytoplasmic membrane proteins (IRMPs) of 32 and 36 kDa in S. epidermidis. Other CNS strains only appeared to express a single IRMP, which varied in molecular mass from 32 to 36 kDa. Whilst the IRMP in these CNS strains showed considerable antigenic homology with the 32 kDa IRMP, the S. warneri IRMP showed cross-reactivity with both the 32 and 36 kDa IRMPs of S. epidermidis. Immunoblotting experiments revealed that whilst the CNS cell wall proteins were poorly immunogenic, the IRMPs were the immunodominant CNS protein antigens, reacting strongly with antibodies present in HPD. This finding provides evidence to suggest that the IRMPs are expressed in vivo during infection.
从持续性非卧床腹膜透析相关性腹膜炎患者中分离出凝固酶阴性葡萄球菌（CNS）菌株，包括表皮葡萄球菌、人葡萄球菌、沃氏葡萄球菌、模仿葡萄球菌、头状葡萄球菌、溶血葡萄球菌和腐生葡萄球菌。比较了在营养肉汤（NB）或混合人腹膜透析液（HPD）中培养的CNS菌株的细胞壁和细胞质膜蛋白谱。在细胞壁和膜蛋白谱方面均观察到一些种间差异。然而，在HPD中生长的CNS菌株的细胞壁蛋白谱与在NB中培养的菌株明显不同。在HPD中生长导致细胞壁相关蛋白总数显著减少，但根据菌种不同，在40 - 56 kDa范围内有多达三种抗原相关蛋白占主导地位。在HPD中生长还导致表皮葡萄球菌诱导产生两种分子量分别为32 kDa和36 kDa的铁抑制性细胞质膜蛋白（IRMPs）。其他CNS菌株似乎只表达一种IRMP，其分子量在32至36 kDa之间变化。虽然这些CNS菌株中的IRMP与32 kDa的IRMP具有相当的抗原同源性，但沃氏葡萄球菌的IRMP与表皮葡萄球菌的32 kDa和36 kDa的IRMP均有交叉反应。免疫印迹实验表明，虽然CNS细胞壁蛋白的免疫原性较差，但IRMPs是CNS的主要免疫蛋白抗原，与HPD中存在的抗体强烈反应。这一发现为IRMPs在感染期间在体内表达提供了证据。
