Lu Yan-Hua, Wang Zheng-Tao, Wei Dong-Zhi, Xiang Hai-Bo
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China.
J Enzyme Inhib Med Chem. 2007 Aug;22(4):433-8. doi: 10.1080/14756360601141562.
The whitening effects of the flavonoid constituents of Alpinia officinarum Hance were investigated on melanin biosynthesis in B 16 mouse melanoma cells, tyrosinase inhibition and UV absorption. The melanin content was reduced to 1.276 microg /10(5) cell for flavonoid mixture and 1.161 microg /10(5) cell for galangin while the melanin control was 1.632 microg/10(5) cell. Both flavonoid mixture and galangin reduced melanin production with an inhibition of 21.81% and 28.86% at a concentration of 26.5 microg/mL and 29 microg/mL (107.4 microM), respectively. Tyrosinase inhibition by the flavonoid mixture and galangin were higher at lower concentrations and galangin showed competitive inhibition at a concentration less than 21.23 microg/mL which was soluble. In addition, the flavonoid mixture and galangin showed a broad absorption band at 270 approximately 290 nm related to the UV-B area. These observations suggest that galangin may be a whitening agent and a promising candidate for prevention of skin cancer. This is the first full scale report on the evaluation of the whitening effect of galangin.
研究了高良姜黄酮类成分对B16小鼠黑色素瘤细胞黑色素生物合成、酪氨酸酶抑制及紫外线吸收的美白效果。黄酮类混合物处理后黑色素含量降至1.276μg/10⁵细胞，高良姜素处理后降至1.161μg/10⁵细胞，而黑色素对照为1.632μg/10⁵细胞。黄酮类混合物和高良姜素在浓度分别为26.5μg/mL和29μg/mL（107.4μM）时，均减少黑色素生成，抑制率分别为21.81%和28.86%。黄酮类混合物和高良姜素在较低浓度时对酪氨酸酶的抑制作用更强，高良姜素在浓度小于21.23μg/mL（可溶）时表现出竞争性抑制。此外，黄酮类混合物和高良姜素在270～290nm处呈现与UV - B区域相关的宽吸收带。这些观察结果表明，高良姜素可能是一种美白剂，也是预防皮肤癌的有潜力候选物。这是关于高良姜素美白效果评估的首份全面报告。
