Chakrabarty Anindita, Tranguch Susanne, Daikoku Takiko, Jensen Kevin, Furneaux Henry, Dey Sudhansu K
Department of Pediatrics, Vanderbilt University Medical Center, 1161 21st Avenue South, Nashville, TN 37232, USA.
Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):15144-9. doi: 10.1073/pnas.0705917104. Epub 2007 Sep 11.
The implantation process is complex, requiring reciprocal interactions between implantation-competent blastocysts and the receptive uterus. Because microRNAs (miRNAs) have major roles in regulating gene expression, we speculated that they participate in directing the highly regulated spatiotemporally expressed genetic network during implantation. Here, we show that two miRNAs, mmu-miR-101a and mmu-miR-199a*, are spatiotemporally expressed in the mouse uterus during implantation coincident with expression of cyclooxygenase-2, a gene critical for implantation. More interestingly, our in vitro gain- and loss-of-function experiments show that cyclooxygenase-2 expression is posttranscriptionally regulated by these two miRNAs. We report on miRNA-mediated regulation of uterine gene expression in the context of implantation. We believe that many other critical genes related to this process are also regulated by miRNAs. Thus, elucidating the physiological roles of uterine miRNAs will help us better understand the genetic control of implantation, the gateway to a successful pregnancy.
着床过程很复杂，需要具备着床能力的囊胚与处于接受态的子宫之间进行相互作用。由于微小RNA（miRNA）在调节基因表达中起主要作用，我们推测它们参与指导着床过程中高度有序的时空表达基因网络。在此，我们表明，两种miRNA，即小鼠miR-101a和小鼠miR-199a*，在小鼠子宫着床期间呈时空表达，这与环氧化酶-2的表达一致，环氧化酶-2是着床关键基因。更有趣的是，我们的体外功能获得和功能缺失实验表明，环氧化酶-2的表达受这两种miRNA的转录后调控。我们报道了在着床背景下miRNA介导的子宫基因表达调控。我们相信，与这一过程相关的许多其他关键基因也受miRNA调控。因此，阐明子宫miRNA的生理作用将有助于我们更好地理解着床的基因控制，而着床是成功妊娠的关键环节。
