Gupta Prasoon, Yadav Dinesh Kumar, Siripurapu Kiran Babu, Palit Guatam, Maurya Rakesh
Division of Medicinal and Process Chemistry, Central Drug Research Institute, Lucknow, India.
J Nat Prod. 2007 Sep;70(9):1410-6. doi: 10.1021/np0700164. Epub 2007 Sep 13.
Three new compounds, ocimumosides A (1) and B (2) and ocimarin (3), were isolated from an extract of the leaves of holy basil (Ocimum sanctum), together with eight known substances, apigenin, apigenin-7-O-beta-D-glucopyranoside, apigenin-7-O-beta-D-glucuronic acid ( 4), apigenin-7- O-beta- d-glucuronic acid 6''-methyl ester, luteolin-7-O-beta-D-glucuronic acid 6''-methyl ester, luteolin-7-O-beta-D-glucopyranoside, luteolin-5-O-beta-D-glucopyranoside, and 4-allyl-1-O-beta-D-glucopyronosyl-2-hydroxybenzene (5), and two known cerebrosides. The structures of the new compounds were determined on the basis of extensive 1D and 2D NMR spectroscopic analysis. The new compounds (1- 3) and the known compounds 4 and 5 were screened at a dose of 40 mg/kg body weight for acute stress-induced biochemical changes in male Sprague-Dawley rats. Compound 1 displayed promising antistress effects by normalizing hyperglycemia, plasma corticosterone, plasma creatine kinase, and adrenal hypertrophy. Compounds 2 and 5 were also effective in normalizing most of these stress parameters. In contrast, compounds 3 and 4 were ineffective in normalizing any of these effects.
从圣罗勒（Ocimum sanctum）叶提取物中分离出三种新化合物，即罗勒苷A（1）、罗勒苷B（2）和罗勒苦素（3），同时还分离出八种已知物质，芹菜素、芹菜素-7-O-β-D-吡喃葡萄糖苷、芹菜素-7-O-β-D-葡萄糖醛酸（4）、芹菜素-7-O-β-D-葡萄糖醛酸6''-甲酯、木犀草素-7-O-β-D-葡萄糖醛酸6''-甲酯、木犀草素-7-O-β-D-吡喃葡萄糖苷、木犀草素-5-O-β-D-吡喃葡萄糖苷和4-烯丙基-1-O-β-D-吡喃葡萄糖基-2-羟基苯（5），以及两种已知的脑苷脂。通过广泛的一维和二维核磁共振光谱分析确定了新化合物的结构。以40mg/kg体重的剂量对新化合物（1-3）以及已知化合物4和5进行筛选，以观察其对雄性Sprague-Dawley大鼠急性应激诱导的生化变化的影响。化合物1通过使高血糖、血浆皮质酮、血浆肌酸激酶和肾上腺肥大恢复正常，显示出有前景的抗应激作用。化合物2和5在使大多数这些应激参数恢复正常方面也有效。相比之下，化合物3和4在使这些效应中的任何一种恢复正常方面均无效。
