Ytting H, Christensen I J, Thiel S, Jensenius J C, Svendsen M N, Nielsen L, Lottenburger T, Nielsen H J
Department of Surgical Gastroenterology, Hvidovre Hospital, University of Copenhagen, Copenhagen, Denmark.
Scand J Immunol. 2007 Oct;66(4):458-64. doi: 10.1111/j.1365-3083.2007.01991.x.
Mannan-binding lectin (MBL) and MBL-associated serine protease 2 (MASP-2) are central components of the MBL pathway of complement activation, and may have potential as clinical biomarkers in colorectal cancer (CRC). Prior to clinical usage, knowledge of the biological variations of the molecules is needed. We here investigate variations of MBL and MASP-2 in healthy persons over time and in relation to gender, age and physical activity. MBL and MASP-2 concentrations were determined in serum from healthy adults over a 3-week period and this was repeated 6 months later (n = 32); during a 24-h period (n = 16); and in relation to physical exercise (n = 14). Concentrations in serum and plasma were compared (n = 198). No significant variation over 6 months and no circadian variation was found for MBL (P = 0.39 and P = 0.34 respectively) or MASP-2 (P = 0.54 and P = 0.55). Physical exercise did not affect the levels (P > 0.8). Serum and plasma levels were only marginally different, and were independent of age and gender. Circulating levels of MBL and MASP-2 are stable over time in healthy individuals, which is advantageous for their potential application as biomarkers.
甘露聚糖结合凝集素（MBL）和MBL相关丝氨酸蛋白酶2（MASP - 2）是补体激活MBL途径的核心成分，在结直肠癌（CRC）中可能具有作为临床生物标志物的潜力。在临床应用之前，需要了解这些分子的生物学变异情况。我们在此研究健康人MBL和MASP - 2随时间的变化以及与性别、年龄和体力活动的关系。在3周内测定了健康成年人血清中的MBL和MASP - 2浓度，并在6个月后重复进行（n = 32）；在24小时内进行了测定（n = 16）；还研究了与体育锻炼的关系（n = 14）。比较了血清和血浆中的浓度（n = 198）。未发现MBL（分别为P = 0.39和P = 0.34）或MASP - 2（P = 0.54和P = 0.55）在6个月内有显著变化，也未发现昼夜变化。体育锻炼未影响其水平（P > 0.8）。血清和血浆水平仅有微小差异，且与年龄和性别无关。健康个体中MBL和MASP - 2的循环水平随时间稳定，这有利于它们作为生物标志物的潜在应用。
