Kerur N, Jhala M K, Joshi C G
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388 001, Gujarat State, India.
Res Vet Sci. 2008 Aug;85(1):176-83. doi: 10.1016/j.rvsc.2007.07.007. Epub 2007 Sep 11.
Peste des petits ruminants (PPR) is an important viral disease of sheep and goats, endemic in India. The study was undertaken to characterize the local PPRV by sequencing fusion (F) protein and nucleoprotein (N) gene segments and phylogenetic analysis, so as to focus on genetic variation in the field viruses. Selected regions of PPRV genome were amplified from clinical samples collected from 32 sheep and goats by RT-PCR and the resulting amplicons were sequenced for phylogenetic analysis. The phylogenetic tree based on the 322bp F gene sequences of PPRV from five different locations clustered them into lineage 4 along with other Asian isolates. While the 425bp N gene sequences revealed a different pattern of branching, yielding three distinct clusters for Nigerian, Turkey and Indian isolates. Thus, classification of PPRV into lineages based on the N gene sequences appeared to yield better picture of molecular epidemiology for PPRV.
小反刍兽疫（PPR）是绵羊和山羊的一种重要病毒性疾病，在印度呈地方流行。本研究旨在通过对融合（F）蛋白和核蛋白（N）基因片段进行测序及系统发育分析来鉴定当地的小反刍兽疫病毒（PPRV），从而关注野外病毒的遗传变异。从32只绵羊和山羊采集的临床样本中，通过逆转录聚合酶链反应（RT-PCR）扩增PPRV基因组的选定区域，并对所得扩增子进行测序以进行系统发育分析。基于来自五个不同地点的PPRV的322bp F基因序列构建的系统发育树将它们与其他亚洲分离株一起聚类到谱系4中。而425bp N基因序列显示出不同的分支模式，尼日利亚、土耳其和印度分离株形成三个不同的簇。因此，基于N基因序列将PPRV分类似乎能更好地呈现PPRV的分子流行病学情况。
