Vardakou Maria, Nueno Palop Carmen, Gasson Michael, Narbad Arjan, Christakopoulos Paul
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str., Zografou Campus, 15780 Athens, Greece; Institute of Food Research, Norwich Research Park, Colney, NR4 7UA Norwich, UK.
Int J Biol Macromol. 2007 Dec 1;41(5):584-9. doi: 10.1016/j.ijbiomac.2007.07.017. Epub 2007 Aug 3.
In vitro fermentations were carried out by using a model of the human colon to stimulate microbial activities of gut bacteria. The model consisted of a three-stage culture system. Bacterial populations were evaluated under the effect of three types of arabinoxylan, a nonstarch polysaccharide derived from wheat, the water-unextractable arabinoxylan fraction (WU-AX), WU-AX pretreated with exogenous xylanase and the soluble water-extractable arabinoxylan fraction (WE-AX). The xylanase pretreated (WU-AX) had a stimulatory effect upon colonic bifidobacteria throughout all three vessels. Counts of Bacteroides spp. and Clostridium spp. were also both significantly reduced. Addition of the WU-AX substrates to the first vessel resulted in induction of bacterial synthesis of extracellular hydrolytic enzymes xylanase and ferulic acid esterase which are both required for bacterial metabolism of WU-AX; this induction was significantly greater with the xylanase treated WU-AX.
通过使用人体结肠模型进行体外发酵，以刺激肠道细菌的微生物活性。该模型由一个三级培养系统组成。在三种阿拉伯木聚糖（一种从小麦中提取的非淀粉多糖）、水不溶性阿拉伯木聚糖部分（WU-AX）、用外源木聚糖酶预处理的WU-AX和可溶性水提取阿拉伯木聚糖部分（WE-AX）的作用下，对细菌群体进行了评估。木聚糖酶预处理的（WU-AX）在所有三个容器中对结肠双歧杆菌都有刺激作用。拟杆菌属和梭菌属的数量也都显著减少。将WU-AX底物添加到第一个容器中导致诱导细菌合成细胞外水解酶木聚糖酶和阿魏酸酯酶，这两种酶都是WU-AX细菌代谢所必需的；用木聚糖酶处理的WU-AX诱导作用明显更强。
