Téclès Odile, Laurent Patrick, Aubut Virginie, About Imad
Laboratoire IMEB-ERT 30, Faculté d'Odontologie, Université de la Méditerranée, 27 Boulevard Jean Moulin, 13355 Marseille Cedex 05, France.
J Biomed Mater Res B Appl Biomater. 2008 Apr;85(1):180-7. doi: 10.1002/jbm.b.30933.
In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with biomaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR(R) or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of biomaterials on this process.
在之前的一项研究中，基于人未成熟第三磨牙的体外全牙培养模型，我们证明了血管周祖细胞在牙髓暴露后能够增殖并迁移至损伤部位。在这项研究中，我们研究了使用修复牙科中经典使用的生物材料进行直接盖髓后细胞的分化情况。用氢氧化钙XR(R)或MTA进行直接盖髓后的组织学染色显示，早期和逐渐形成的矿化灶中含有BrdU标记的隔离细胞。通过免疫组织化学（I型胶原蛋白、牙本质涎蛋白和巢蛋白）对基质和隔离细胞进行分子表征，清楚地表明这些区域具有由成牙本质细胞样细胞形成的修复性牙本质矿化基质的共同特征。这重现了在体内应用这些材料后牙髓反应的一些特征，并证明全牙培养模型重现了体内牙本质再生早期步骤的一部分。其未来的发展可能有助于研究生物材料对这一过程的影响。
