Tran An Thi Loc, Sukajintanakarn Charnsak, Senawongse Pisol, Sritanaudomchai Hathaitip, Ruangsawasdi Nisarat, Lapthanasupkul Puangwan, Kitkumthorn Nakarin, Monmaturapoj Naruporn, Khamsut Chutikarn, Naruphontjirakul Parichart, Pongprueksa Pong
Dental Biomaterials Science Program, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University, Bangkok, Thailand.
Eur J Dent. 2023 Oct;17(4):1120-1128. doi: 10.1055/s-0042-1758789. Epub 2023 Feb 22.
To evaluate the potential of modified bioactive glasses containing lithium and zinc as pulp capping materials by investigating the odontogenic differentiation and mineralization response in the tooth culture model.
Lithium- and zinc-containing bioactive glasses (45S5.1Li, 45S5.5Li, 45S5.1Zn, 45S5.5Zn, 45S5.1Zn sol-gel, and 45S5.5Zn sol-gel), fibrinogen-thrombin, and biodentine were prepared to assess gene expression at 0, 30 minutes, 1 hour, 12 hours, and 1 day and gene expression at 0, 3, 7, and 14 days in stem cells from human exfoliated deciduous teeth (SHEDs) using qRT-PCR. The experimental bioactive glasses incorporated with fibrinogen-thrombin and biodentine were placed on the pulpal tissue in the tooth culture model. Histology and immunohistochemistry were analyzed at 2 weeks and 4 weeks.
gene expression for all experimental groups was significantly higher than the control at 12 hours. The gene expression for all experimental groups was significantly higher than the control at 14 days. The presence of mineralization foci was significantly higher at 4 weeks for the modified bioactive glasses 45S5.5Zn, 45S5.1Zn sol-gel, and 45S5.5Zn sol-gel as well as Biodentine compared with the fibrinogen-thrombin control.
Lithium and zinccontaining bioactive glasses increased and gene expression in SHEDs and can potentially enhance pulp mineralization and regeneration. Zinccontaining bioactive glasses are a promising candidate to be used as pulp capping materials.
通过研究牙齿培养模型中的牙源性分化和矿化反应，评估含锂和锌的改性生物活性玻璃作为牙髓盖髓材料的潜力。
制备含锂和锌的生物活性玻璃（45S5.1Li、45S5.5Li、45S5.1Zn、45S5.5Zn、45S5.1Zn溶胶-凝胶和45S5.5Zn溶胶-凝胶）、纤维蛋白原-凝血酶和生物活性牙本质，使用qRT-PCR评估人乳牙脱落干细胞（SHEDs）在0、30分钟、1小时、12小时、1天以及0、3、7和14天的基因表达。将与纤维蛋白原-凝血酶和生物活性牙本质结合的实验性生物活性玻璃放置在牙齿培养模型的牙髓组织上。在2周和4周时进行组织学和免疫组织化学分析。
所有实验组在12小时时的基因表达均显著高于对照组。所有实验组在14天时的基因表达均显著高于对照组。与纤维蛋白原-凝血酶对照组相比，改性生物活性玻璃45S5.5Zn、45S5.1Zn溶胶-凝胶和45S5.5Zn溶胶-凝胶以及生物活性牙本质在4周时矿化灶的存在显著更高。
含锂和锌的生物活性玻璃增加了SHEDs中的基因表达，可能增强牙髓矿化和再生。含锌的生物活性玻璃是用作牙髓盖髓材料的有前途的候选材料。
