Liu Qun, Cai Gan
Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China.
Zhong Xi Yi Jie He Xue Bao. 2007 Sep;5(5):555-8.
To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome.
Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected.
The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P<0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P<0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P<0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P<0.05).
The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.
研究脾虚证大鼠模型中神经肽含量的变化。
将大鼠随机分为三组：正常组、未治疗组和治疗组。每组10只大鼠。未治疗组和治疗组大鼠通过灌胃给予大黄浓缩液诱导脾虚证。治疗组大鼠灌胃给予四君子汤，一种传统的健脾益气复方中药。正常组和未治疗组大鼠灌胃给予生理盐水。采用放射免疫分析法检测下丘脑和结肠中生长抑素（SS）和胆囊收缩素-8（CCK-8）的含量，并检测尿中D-木糖含量。
未治疗组下丘脑CCK-8含量高于正常组（P<0.05）。未治疗组结肠中SS和CCK-8含量高于正常组（P<0.05）。未治疗组结肠中CCK-8含量低于治疗组（P<0.05）。未治疗组D-木糖排泄率低于正常组（P<0.05）。
脾虚证大鼠神经内分泌调节紊乱可能是中医“脾虚及脾不健运”的主要原因。
