Gao Xiao-Ling, Guo Wen-Feng, Li Ru-Liu, Chen Wei-Wen
Piwei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
Zhong Yao Cai. 2009 Aug;32(8):1242-5.
To observe the effects of Sijunzi Decoction on D-xylose excretion rate and ATP content in the mucosa membranes of small intestines of rats with spleen deficiency.
Spleen deficiency model rats were made by reserpine injection. D-xylose excretion rate was measured with p-bromoaniline method, and the ATP content of small intestines mucosa was detected with bioluminescence method. The correlation between D-xylose excretion rate and ATP content of mucosa was also analyzed.
Rats' body weight and D-xylose excretion rate decreased after reserpine injection (P < 0.01, vs control group), but increased after treated with Sijunzi Decoction (P < 0.05, vs model group). The ATP content of mucosa showed no significant difference between model group and control group. There was obviously positive correlation between the change of urine's D-xylose excretion rate and mucosa ATP content.
Sijunzi Decoction has the activity of improving xylose absorption in spleen deficiency rats, but no obvious effect on their mucosa ATP content. The reducing of urine's D-xylose excretion rate in spleen deficiency rats is accompanied with the decrease of mucosa ATP content.
观察四君子汤对脾虚大鼠D-木糖排泄率及小肠黏膜ATP含量的影响。
采用利血平注射法制备脾虚模型大鼠。用对溴苯胺法测定D-木糖排泄率，用生物发光法检测小肠黏膜ATP含量。并分析D-木糖排泄率与黏膜ATP含量之间的相关性。
利血平注射后大鼠体重及D-木糖排泄率降低（与对照组相比，P<0.01），但四君子汤治疗后升高（与模型组相比，P<0.05）。模型组与对照组黏膜ATP含量差异无统计学意义。尿D-木糖排泄率变化与黏膜ATP含量之间存在明显正相关。
四君子汤具有改善脾虚大鼠木糖吸收的作用，但对其黏膜ATP含量无明显影响。脾虚大鼠尿D-木糖排泄率降低伴随着黏膜ATP含量的减少。
