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显微镜中的自适应光学技术。

Adaptive optics in microscopy.

作者信息

Booth Martin J

机构信息

Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.

出版信息

Philos Trans A Math Phys Eng Sci. 2007 Dec 15;365(1861):2829-43. doi: 10.1098/rsta.2007.0013.

DOI:10.1098/rsta.2007.0013
PMID:17855218
Abstract

The imaging properties of optical microscopes are often compromised by aberrations that reduce image resolution and contrast. Adaptive optics technology has been employed in various systems to correct these aberrations and restore performance. This has required various departures from the traditional adaptive optics schemes that are used in astronomy. This review discusses the sources of aberrations, their effects and their correction with adaptive optics, particularly in confocal and two-photon microscopes. Different methods of wavefront sensing, indirect aberration measurement and aberration correction devices are discussed. Applications of adaptive optics in the related areas of optical data storage, optical tweezers and micro/nanofabrication are also reviewed.

摘要

光学显微镜的成像特性常常受到像差的影响,这些像差会降低图像分辨率和对比度。自适应光学技术已应用于各种系统中,以校正这些像差并恢复性能。这需要在多个方面偏离天文学中使用的传统自适应光学方案。本综述讨论了像差的来源、其影响以及如何用自适应光学进行校正,特别是在共聚焦显微镜和双光子显微镜中。文中还讨论了不同的波前传感方法、间接像差测量方法和像差校正装置。此外,还综述了自适应光学在光数据存储、光镊和微纳制造等相关领域的应用。

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Philos Trans A Math Phys Eng Sci. 2007 Dec 15;365(1861):2829-43. doi: 10.1098/rsta.2007.0013.
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