Morales M, Fifková E
Department of Psychology, University of Colorado, Boulder 80309.
Cell Tissue Res. 1991 Sep;265(3):415-23. doi: 10.1007/BF00340864.
We studied the solubility properties of brain acetylated alpha-tubulin, as well as the localization of this tubulin in brain tissue. Endogenous unpolymerized tubulin and cytoskeletal tubulin were fractionated after brain Triton-solubilization. Using the immunoblotting technique, we found that acetylated alpha-tubulin was recovered in the cytoskeletal fraction, and that most (92%) of the acetylated microtubules of this fraction were depolymerized by cold/Ca2+ treatment. In another set of experiments, axonal and soma-dendritic preparations were found to have equivalent amounts of acetylated alpha-tubulin. By immunogold electron microscopy, we established that acetylated microtubules are widely distributed in dendrites of the central nervous system.
我们研究了脑乙酰化α-微管蛋白的溶解性特性，以及该微管蛋白在脑组织中的定位。脑经曲拉通溶解后，对内源性未聚合微管蛋白和细胞骨架微管蛋白进行了分级分离。使用免疫印迹技术，我们发现乙酰化α-微管蛋白存在于细胞骨架组分中，并且该组分中大部分（92%）的乙酰化微管经冷/Ca2+处理后会解聚。在另一组实验中，发现轴突和胞体-树突制剂中乙酰化α-微管蛋白的含量相当。通过免疫金电子显微镜，我们确定乙酰化微管广泛分布于中枢神经系统的树突中。
