Holmes G L, Thompson J L, Huh K, Holmes C, Carl G F
Department of Neurology, Children's Hospital, Boston, MA 02115.
Brain Res Dev Brain Res. 1991 Dec 17;64(1-2):47-56. doi: 10.1016/0165-3806(91)90208-z.
To study the hypothesis that neural transplantations can alter seizure susceptibility in a chronic animal model of epilepsy 260 immature rats (30- to 32-days-old) were administered a convulsant dosage of kainic acid (KA). Ten days later rats that had severe seizures following KA received either bilateral intracerebroventricular transplants of hippocampal (n = 27), neocortical (n = 29), cerebellar (n = 30), or locus ceruleus (n = 32) tissue, or underwent sham transplantation (n = 66). Spontaneous seizure frequency was assessed for 230 days following which the rats underwent entorhinal kindling. The percentage of rats developing spontaneous recurrent seizures was similar in the 4 transplant groups and the sham-operated controls. Rats receiving hippocampal and locus ceruleus transplants had fewer spontaneous seizures than the sham-operated controls or other transplant groups. However, there were no differences in afterdischarge thresholds or kindling rates in the 5 groups. This study demonstrates that the anticonvulsant effects of neural transplants, using this animal model are mild. Tissue type of the graft appears to be an important variable in the alteration of seizure frequency.
为研究神经移植能否改变慢性癫痫动物模型的癫痫易感性这一假说，对260只未成熟大鼠（30至32日龄）给予惊厥剂量的 kainic 酸（KA）。10天后，KA 给药后出现严重癫痫发作的大鼠，分别接受双侧海马（n = 27）、新皮质（n = 29）、小脑（n = 30）或蓝斑（n = 32）组织的脑室内移植，或进行假移植（n = 66）。评估230天的自发癫痫发作频率，之后对大鼠进行内嗅皮质点燃。4个移植组和假手术对照组中出现自发反复癫痫发作的大鼠百分比相似。接受海马和蓝斑移植的大鼠比假手术对照组或其他移植组的自发癫痫发作更少。然而，5组之间的后放电阈值或点燃率没有差异。本研究表明，使用该动物模型时神经移植的抗惊厥作用轻微。移植组织类型似乎是癫痫发作频率改变的一个重要变量。
