McClure Evelyn L, Wong Charles S
Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
J Chromatogr A. 2007 Oct 26;1169(1-2):53-62. doi: 10.1016/j.chroma.2007.08.062. Epub 2007 Sep 1.
In this work, we optimize a solid phase microextraction (SPME) method for the simultaneous collection of antibiotics (sulfonamides, macrolides, and trimethoprim) present in wastewaters. The performance of the SPME method is compared to a solid phase extraction (SPE) method. Analytes in both cases were quantified by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) with electrospray ionization. The advantages offered by SPME in this application are: decreased sample volume requirements, ease of sample processing and extraction, decreased cost, and most importantly, elimination of electrospray matrix effects. Despite having higher limits of quantification (16-1380 ng/L in influent and 35-260 ng/L in effluent), nearly all of the compounds found to be present in Edmonton Gold Bar wastewater by SPE were measurable by SPME (i.e., sulfamethoxazole, trimethoprim, erythromycin, and clarithromycin), with values similar to those obtained using the former method. Limits of quantification for the SPE method for the measured compounds were 4.7-15 ng/L and 0.86-6.1 ng/L for influent and effluent, respectively.
在本研究中，我们优化了一种固相微萃取（SPME）方法，用于同时收集废水中存在的抗生素（磺胺类、大环内酯类和甲氧苄啶）。将SPME方法的性能与固相萃取（SPE）方法进行了比较。两种情况下的分析物均通过带电喷雾电离的高效液相色谱-串联质谱（HPLC-MS-MS）进行定量。SPME在该应用中提供的优势包括：减少样品体积需求、易于样品处理和萃取、降低成本，以及最重要的是，消除电喷雾基质效应。尽管定量限较高（进水为16 - 1380 ng/L，出水为35 - 260 ng/L），但通过SPE在埃德蒙顿金条废水中发现的几乎所有化合物都能用SPME检测到（即磺胺甲恶唑、甲氧苄啶、红霉素和克拉霉素），其值与使用前一种方法获得的值相似。所测化合物的SPE方法定量限分别为进水4.7 - 15 ng/L和出水0.86 - 6.1 ng/L。
