Macedo Carla Isabel, Christofoletti Alice, Munford Veridiana, Rácz Maria Lúcia
Institute Pasteur São Paulo, SP Brasil.
Rev Soc Bras Med Trop. 2007 Jul-Aug;40(4):381-4. doi: 10.1590/s0037-86822007000400001.
A total of 123 stool specimens collected in Teresina, Piauí between 1994 and 1996, from 0 to 2-year-old children with diarrhea, were used for this study. Molecular characterization of the G and P rotavirus genotypes was performed using the reverse transcriptase polymerase chain reaction. The following results were obtained for the P genotypes: P[8] (17. 1%), P[1] (4. 9%), P[4] (3. 3%), P[6, M37] (2. 4%) and mixtures (27. 6%). The P[1]+P[8] mixture was found in 19. 5% of the samples. For the G genotypes, the results were: G1 (25. 2%), G5 (13. 8%), G2 (2. 5%), G4 (2. 5%), G9 (0. 8%) and mixtures (41. 5%). G1+G5 was the mixture most frequently found (12. 1%). Our results showed unusual combinations such as P[1]G5 and P[1]+P[8]G5. The high percentage of mixtures and unusual combinations containing mixtures of human and animal rotavirus genotypes strongly suggests the possibility of gene reassortment and interspecies transmission.
1994年至1996年期间，在皮奥伊州特雷西纳收集了123份粪便标本，这些标本来自0至2岁腹泻儿童，用于本研究。使用逆转录聚合酶链反应对G和P轮状病毒基因型进行分子特征分析。P基因型的结果如下：P[8]（17.1%）、P[1]（4.9%）、P[4]（3.3%）、P[6，M37]（2.4%）和混合基因型（27.6%）。在19.5%的样本中发现了P[1]+P[8]混合基因型。对于G基因型，结果如下：G1（25.2%）、G5（13.8%）、G2（2.5%）、G4（2.5%）、G9（0.8%）和混合基因型（41.5%）。G1+G5是最常见的混合基因型（12.1%）。我们的结果显示了不寻常的组合，如P[1]G5和P[1]+P[8]G5。混合基因型以及包含人和动物轮状病毒基因型混合的不寻常组合的高比例强烈表明基因重配和种间传播的可能性。
