Yang S S, Ling M Y
Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, 10764, Republic of China.
Biotechnol Bioeng. 1989 Mar;33(8):1021-8. doi: 10.1002/bit.260330811.
For saving energy in antibiotic production and reducing the amount of agricultural wastes, solid state fermentation was used in this study to produce tetracycline with sweet potato residue by Streptomyces viridifaciens ATCC 11989. It was found that the optimal media for tetracycline production were sweet potato residue 100 g, organic nitrogen (rice bran, wheat bran, or peanut meal) 20 g, (NH(4))(2)SO(4) 2.4 g, KH(2)PO(4) 0.4 g, CaCO(3) 1.8 g, NaCl 0.6 g, MgCl(2) 0.8 g, soluble starch 10 g, methionine 0.2 g, histidine 0.8 g, and monosodium glutamate 1.6 g with initial moisture content 68-72%, and initial pH 5.8-6.0. Each gram of dry weight substrate was inoculated with 1.0 x 10(8) conidia and incubated at 26 degrees C for 5-7 days, producing 4720 mug of total tetracycline equivalent potency. When incubated at 26 degrees C with the initial moisture content 68%, the conidia in solid media germinated on the second day, mycelia grew abundantly on the third day and reached stationary phase on the sixth day. The antibiotic production was consistent with the morphogenesis of S. viridifaciens: activity could be detected on the third day, had the maximal potency on the sixth day, and decreased slightly on the tenth day. (11-3-88 tly).
为了在抗生素生产中节约能源并减少农业废弃物的数量，本研究采用固态发酵法，利用甘薯渣由绿色链霉菌ATCC 11989生产四环素。结果发现，四环素生产的最佳培养基为：甘薯渣100克、有机氮（米糠、麦麸或花生粕）20克、硫酸铵2.4克、磷酸二氢钾0.4克、碳酸钙1.8克、氯化钠0.6克、氯化镁0.8克、可溶性淀粉10克、蛋氨酸0.2克、组氨酸0.8克、味精1.6克，初始水分含量68 - 72%，初始pH值5.8 - 6.0。每克干重底物接种1.0×10⁸个分生孢子，在26℃下培养5 - 7天，产生4720微克总四环素当量效价。当在26℃、初始水分含量68%的条件下培养时，固体培养基中的分生孢子在第二天萌发，菌丝体在第三天大量生长并在第六天达到稳定期。抗生素的产生与绿色链霉菌的形态发生一致：在第三天可检测到活性，在第六天效价最高，在第十天略有下降。（11 - 3 - 88 tly）
