Zhang Wen-yi, Jiang Jia-fu, Yao Kun, Wu Xiao-ming, Zuo Shu-ging, Zhan Lin, Chu Chen-yi, Zhao Qiu-min, Zhang Pan-he, Yang Hong, Cao Wu-chun
State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing 100071, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2007 May;28(5):482-6.
To confirm the existence of Amur-like viruses in Apodemus peninsulae in China, and to understand the molecular characteristics of these viruses.
Total RNA was extracted from lungs of A. peninsulae captured in Jilin of Northeast China with Trizol reagent. Complete S and partial M segments of Amur virus were amplified and sequenced. Phylogenetic analyses on multiple nucleotide sequences were performed with the Clustal method and DNASTAR software.
383 bp cDNA of M segment and 1696 bp of S segment of Amur like virus were recovered from lung tissue of A. peninsulae, named JilinAP06. The full-length of its S gene comprised of 1696 nucleotides with ORF including 1287 nucleotides and encoding a protein which comprised 429 amino acids. The phylogenetic analysis of this sample with other hantaviruses revealed that the complete S and partial M segment sequence of JilinAP06 both were closely related to those Amur viruses such as AP63, AP61, AP1371 and AP1168 found in A. peninsulae from Far East region of Russia and B78 strain, Liu strain and H5 strain, which were all from Chinese patients. The complete S and partial M segment sequence of JilinAP06 had only 81.0% identities with the nucleotide sequences of HV prototype 76-118 strain.
Amur-like viruses did exist in A. peninsulae from Northeasern China while A. peninsulae might be the natural reservoir of Amur-like viruses in China and was the important infectious source to HFRS patients which were caused by Amur-like viruses.
证实中国东北棕背䶄中存在类阿穆尔病毒，并了解这些病毒的分子特征。
用Trizol试剂从中国东北吉林省捕获的棕背䶄肺组织中提取总RNA。扩增并测序阿穆尔病毒的完整S片段和部分M片段。采用Clustal法和DNASTAR软件对多个核苷酸序列进行系统发育分析。
从棕背䶄肺组织中获得了类阿穆尔病毒M片段的383 bp cDNA和S片段的1696 bp，命名为吉林AP06。其S基因全长1696个核苷酸，开放阅读框包含1287个核苷酸，编码一个由429个氨基酸组成的蛋白质。该样本与其他汉坦病毒的系统发育分析表明，吉林AP06的完整S片段和部分M片段序列均与在俄罗斯远东地区棕背䶄中发现的阿穆尔病毒如AP63、AP61、AP1371和AP1168以及均来自中国患者的B78株、刘株和H5株密切相关。吉林AP06的完整S片段和部分M片段序列与HV原型76 - 118株的核苷酸序列仅有81.0%的同源性。
中国东北地区的棕背䶄中确实存在类阿穆尔病毒，棕背䶄可能是中国类阿穆尔病毒的自然宿主，是由类阿穆尔病毒引起的肾综合征出血热患者的重要传染源。
