Baek Luck Ju, Kariwa Hiroaki, Lokugamage Kumari, Yoshimatsu Kumiko, Arikawa Jiro, Takashima Ikuo, Kang Ju-Il, Moon Sung Sil, Chung Su Yong, Kim Eun Ju, Kang Hae Ji, Song Ki-Joon, Klein Terry A, Yanagihara Richard, Song Jin-Won
Department of Microbiology, College of Medicine, Institute for Viral Diseases, Korea University, Seoul, Korea.
J Med Virol. 2006 Feb;78(2):290-7. doi: 10.1002/jmv.20538.
Hantaan (HTN) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (HFRS), was first isolated in 1976 from lung tissue of a striped-field mouse (Apodemus agrarius) captured in Songnae-ri, Gyeonggi Province, Republic of Korea. Found primarily in mountainous areas, the Korean field mouse (A. peninsulae) is the second-most dominant field rodent species found throughout Korea. A new hantavirus, designated Soochong (SOO), was isolated in Vero E6 cells from four A. peninsulae captured in August 1997 at Mt. Gyebang in Hongcheon-gun, Mt. Gachil, Inje-gun, Gangwon Province, and in September 1998 at Mt. Deogyu, Muju-gun, Jeollabuk Province. The entire S, M, and L genomic segments of SOO virus, amplified by RT-PCR from lung tissues of seropositive A. peninsulae and from virus-infected Vero E6 cells, diverged from HTN virus (strain 76-118) by 15.6%, 22.8%, and 21.7% at the nucleotide level and 3.5%, 9.5%, and 4.6% at the amino acid level, respectively. Phylogenetic analyses of the nucleotide and deduced amino acid sequences, using the maximum parsimony and neighbor-joining methods, indicated that SOO virus was distinct from A. agrarius-borne HTN virus. SOO virus shared a common ancestry with Amur virus from Far East Russia, as well as with H5 and B78 hantaviruses, previously isolated from HFRS patients in China. Cross-focus-reduction neutralizating antibody tests showed that SOO virus, which is the first hantavirus isolated in cell culture from A. peninsulae, could be classified as a new hantavirus serotype.
汉坦（HTN）病毒是临床上严重的肾综合征出血热（HFRS）的病原体，于1976年首次从韩国京畿道松内里捕获的一只条纹田鼠（黑线姬鼠）的肺组织中分离出来。朝鲜田鼠（A. peninsulae）主要分布在山区，是韩国境内第二大优势田鼠物种。1997年8月在江原道洪川郡的皆骨山、麟蹄郡的加七山捕获的4只朝鲜田鼠以及1998年9月在全罗北道茂朱郡的德羽山捕获的朝鲜田鼠中，从其身上采集的样本在Vero E6细胞中分离出一种新的汉坦病毒，命名为寿城（SOO）病毒。通过RT-PCR从血清阳性的朝鲜田鼠肺组织和病毒感染的Vero E6细胞中扩增出SOO病毒的完整S、M和L基因组片段，这些片段在核苷酸水平上与HTN病毒（76-118株）的差异分别为15.6%、22.8%和21.7%，在氨基酸水平上的差异分别为3.5%、9.5%和4.6%。使用最大简约法和邻接法对核苷酸和推导的氨基酸序列进行系统发育分析表明，SOO病毒与由黑线姬鼠传播的HTN病毒不同。SOO病毒与来自俄罗斯远东地区的阿穆尔病毒以及之前从中国HFRS患者中分离出的H5和B78汉坦病毒有共同的祖先。交叉聚焦减少中和抗体试验表明，SOO病毒是首次从朝鲜田鼠细胞培养物中分离出的汉坦病毒，可被归类为一种新的汉坦病毒血清型。
