Tong Hui, Hong Yuning, Dong Yongqiang, Häussler Matthias, Li Zhen, Lam Jacky W Y, Dong Yuping, Sung Herman H-Y, Williams Ian D, Tang Ben Zhong
Department of Chemistry, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, PR China.
J Phys Chem B. 2007 Oct 11;111(40):11817-23. doi: 10.1021/jp073147m. Epub 2007 Sep 18.
Three functionalized derivatives of tetraphenylethylene (TPE), namely, 1,2-bis(4-methoxyphenyl)-1,2-diphenylethene (1), 1,2-bis(4-hydroxyphenyl)-1,2-diphenylethene (2), and 1,2-bis[4-(3-sulfonatopropoxyl)phenyl]-1,2-diphenylethene sodium salt (3), were synthesized and their fluorescence properties were investigated. All the TPE molecules are nonluminescent in the solution state but are induced to emit efficiently by aggregate formation. This novel process of aggregation-induced emission (AIE) is rationalized to be caused by the restriction of intramolecular rotations of the dye molecules in the aggregate state. The possibility of utilizing the AIE effect for protein detection and quantification is explored using bovine serum albumin (BSA) as a model protein, with salt 3 being found to perform as a stable, sensitive, and selective bioprobe.
合成了四苯乙烯（TPE）的三种功能化衍生物，即1,2-双（4-甲氧基苯基）-1,2-二苯乙烯（1）、1,2-双（4-羟基苯基）-1,2-二苯乙烯（2）和1,2-双[4-（3-磺基丙氧基）苯基]-1,2-二苯乙烯钠盐（3），并研究了它们的荧光性质。所有TPE分子在溶液状态下均无发光现象，但通过聚集形成可诱导其高效发光。这种聚集诱导发光（AIE）的新过程被认为是由聚集态下染料分子的分子内旋转受限引起的。以牛血清白蛋白（BSA）为模型蛋白，探索了利用AIE效应进行蛋白质检测和定量的可能性，发现盐3可作为一种稳定、灵敏且具有选择性的生物探针。
