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褐藻(鹿角菜)提取物的抗氧化特性。

The antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts.

作者信息

Cho Sun-hee, Kang Se-eun, Cho Ji-young, Kim Ah-ram, Park Sun-mee, Hong Yong-Ki, Ahn Dong-Hyun

机构信息

Department of Food Science & Technology, Pukyong National University, Busan, Republic of Korea.

出版信息

J Med Food. 2007 Sep;10(3):479-85. doi: 10.1089/jmf.2006.099.

Abstract

Antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts were evaluated using various antioxidant measurements, i.e., inhibitory effect on thiobarbituric acid-reactive substances (TBARS), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, metal chelating effect, reducing power effect, and total phenolic compounds. When the extraction solvents n-hexane, chloroform, ethanol, and water were compared, the water extract showed the highest yield in extracted mass. Total phenolic compounds were the highest in the ethanol extract, with 127.4 mg/g. The TBARS inhibition of chloroform and ethanol extracts at 10 mg/mL was 90.9% and 80.9%, respectively. DPPH radical scavenging capacity was more than 90% in all extracts at 1 mg/mL. The chloroform extract exhibited the highest metal ion chelating ability of 69.6% at 10 mg/mL. The reducing power was found to be the highest in the ethanol extract at 10 mg/mL, showing an effect similar to ascorbic acid. Thus, the ethanol extract of S. siliquastrum has potential as a natural antioxidant.

摘要

利用各种抗氧化剂测定方法,即对硫代巴比妥酸反应性物质(TBARS)的抑制作用、1,1-二苯基-2-苦基肼(DPPH)自由基清除能力、金属螯合作用、还原能力以及总酚类化合物含量,对褐藻(海带)提取物的抗氧化性能进行了评估。当比较正己烷、氯仿、乙醇和水这几种提取溶剂时,水提取物的提取质量产率最高。乙醇提取物中的总酚类化合物含量最高,为127.4毫克/克。氯仿提取物和乙醇提取物在浓度为10毫克/毫升时对TBARS的抑制率分别为90.9%和80.9%。所有提取物在浓度为1毫克/毫升时对DPPH自由基的清除能力均超过90%。氯仿提取物在浓度为10毫克/毫升时表现出最高的金属离子螯合能力,为69.6%。发现乙醇提取物在浓度为10毫克/毫升时还原能力最高,显示出与抗坏血酸类似的效果。因此,海带乙醇提取物具有作为天然抗氧化剂的潜力。

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