Kilpatrick B A, Huang E S, Pagano J S
J Virol. 1976 Jun;18(3):1095-105. doi: 10.1128/JVI.18.3.1095-1105.1976.
Cleavage of genomes of eleven human, one simian, and one simian-related cytomegalovirus (CMV) isolate by the restriction endonucleases HinD III and EcoR-1 generated reproducible DNA fragments. The size range of CMV DNA fragments as estimated by contour length measurements in comparison with simian virus 40 form II DNA and by coelectrophoresis with EcoR-1 fragments of herpes simplex virus DNA varied between 15 X 10(6) and 0.5 X 10(6) daltons. Comparison of the cleavage products of each isolate in 1% agarose slab gels showed extensive comigration of fragments among the human CMV isolates. In the HinD III digests, three fragment bands comigrated among all human CMV isolates, and six fragments comigrated among most, but not all, human CMV isolates. In the EcoR-1 digests, nine fragment bands comigrated among all human CMV isolates, and five bands comigrated among most, but not all human isolates. Each isolate had a distinctive electrophoretic profile with either HinD III or EcoR-1 digests. No two isolates had identical HinD III or EcoR-1 patterns although some isolates did share more general pattern similarities than others. No clear-cut subgrouping of isolates based on cleavage pattern characteristics could be discerned. Comparison of HinD III and EcoR-1 patterns showed that human isolates differ greatly from nonhuman CMV isolates. HinD III and EcoR-1 digests of each isolate contained both major and minor molar classes of DNA fragments that ranged from about 1 and multiples of 1 M down to about 0.25 M; however, the summed molecular weights for major molar fragments resulting from HinD III or EcoR-1 digests of several isolates closely approximated the molecular weight of 10(8) of the intact genome.
用限制性核酸内切酶HinD III和EcoR-1切割11种人类、1种猿猴及1种与猿猴相关的巨细胞病毒（CMV）分离株的基因组，产生了可重复的DNA片段。通过与猿猴病毒40 II型DNA进行轮廓长度测量，并与单纯疱疹病毒DNA的EcoR-1片段进行共电泳，估计CMV DNA片段的大小范围在15×10⁶至0.5×10⁶道尔顿之间。在1%琼脂糖平板凝胶中比较每种分离株的切割产物，结果显示人类CMV分离株之间的片段有大量共迁移现象。在HinD III消化物中，所有人类CMV分离株中有三条片段带共迁移，大多数（但不是全部）人类CMV分离株中有六条片段带共迁移。在EcoR-1消化物中，所有人类CMV分离株中有九条片段带共迁移，大多数（但不是全部）人类分离株中有五条片段带共迁移。每种分离株用HinD III或EcoR-1消化后都有独特的电泳图谱。尽管有些分离株比其他分离株有更多的一般图谱相似性，但没有两个分离株有相同的HinD III或EcoR-1图谱。基于切割图谱特征无法明确区分分离株的亚组。HinD III和EcoR-1图谱的比较表明，人类分离株与非人类CMV分离株有很大差异。每种分离株的HinD III和EcoR-1消化物都包含主要和次要摩尔类别的DNA片段，范围从约1和1M的倍数到约0.25M；然而，几种分离株的HinD III或EcoR-1消化产生的主要摩尔片段的总分子量与完整基因组的10⁸分子量非常接近。
