在锌离子和巯基化合物存在的情况下对脑心肌炎病毒前体多肽的加工。
Processing of mengovirus precursor polypeptides in the presence of zinc ions and sulfhydryl compounds.
作者信息
Nakai K, Lucas-Lenard J
出版信息
J Virol. 1976 Jun;18(3):918-25. doi: 10.1128/JVI.18.3.918-925.1976.
Abstract
The effect of zinc ions on the post-translational cleavage of mengovirus polypeptides has been examined. The cleavage of the "A" precursor, which gives rise to the capsid proteins, was the most sensitive at concentrations of zinc chloride from 0.1 to 1.0 mM. Beta-mercaptoethanol and dithiothreitol antagonized the zinc-promoted inhibtion of clevage. Our results indicate that zinc ions interfere with the proper folding of the nascent polypeptide precursor rather than inhibit the proteases responsible for the cleavages. Thus, proper folding of mengovirus polypeptide "A" appears to be necessary for subsequent processing by proteases.
摘要
已研究了锌离子对脑心肌炎病毒多肽翻译后切割的影响。产生衣壳蛋白的“A”前体的切割在0.1至1.0 mM的氯化锌浓度下最为敏感。β-巯基乙醇和二硫苏糖醇可拮抗锌促进的切割抑制作用。我们的结果表明，锌离子干扰新生多肽前体的正确折叠，而不是抑制负责切割的蛋白酶。因此，脑心肌炎病毒多肽“A”的正确折叠似乎是蛋白酶后续加工所必需的。
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