Tabanca Nurhayat, Demirci Betul, Crockett Sara L, Başer Kemal Hüsnü Can, Wedge David E
USDA, ARS, NPURU, National Center for Natural Products Research, The University of Mississippi, University, Mississippi 38677, USA.
J Agric Food Chem. 2007 Oct 17;55(21):8430-5. doi: 10.1021/jf071379c. Epub 2007 Sep 26.
Essential oils from three different Asteraceae obtained by hydrodistillation of aerial parts were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). Main compounds obtained from each taxon were found as follows: Arnica longifolia carvacrol 37.3%, alpha-bisabolol 8.2%; Aster hesperius hexadecanoic acid 29.6%, carvacrol 15.2%; and Chrysothamnus nauseosus var. nauseosus beta-phellandrene 22.8% and beta-pinene 19.8%. Essential oils were also evaluated for their antimalarial and antimicrobial activity against human pathogens, and antifungal activities against plant pathogens. No antimalarial and antimicrobial activities against human pathogens were observed. Direct bioautography demonstrated antifungal activity of the essential oils obtained from three Asteraceae taxa and two pure compounds, carvacrol and beta-bisabolol, to the plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides. Subsequent evaluation of antifungal compounds using a 96-well micro-dilution broth assay indicated that alpha-bisabolol showed weak growth inhibition of the plant pathogen Botrytis cinerea after 72 h.
通过对三种不同菊科植物地上部分进行水蒸馏得到的挥发油，采用气相色谱（GC）和气相色谱 - 质谱联用（GC/MS）进行分析。从每个分类单元中获得的主要化合物如下：长叶山金车中的香芹酚占37.3%，α-红没药醇占8.2%；西方紫菀中的十六烷酸占29.6%，香芹酚占15.2%；以及 nauseosus 变种的黄褐兔耳草中的β-水芹烯占22.8%，β-蒎烯占19.8%。还评估了这些挥发油对人类病原体的抗疟疾和抗菌活性，以及对植物病原体的抗真菌活性。未观察到对人类病原体的抗疟疾和抗菌活性。直接生物自显影显示，从三种菊科分类单元以及两种纯化合物香芹酚和β-红没药醇中获得的挥发油对植物病原体尖孢炭疽菌、草莓炭疽菌和胶孢炭疽菌具有抗真菌活性。随后使用96孔微量稀释肉汤试验对抗真菌化合物进行评估，结果表明α-红没药醇在72小时后对植物病原体灰葡萄孢菌显示出较弱的生长抑制作用。
