Lucivero G, D'Addario V, Tannoia N, Dell'Osso A, Gambatesa V, Lopalco P L, Cagnazzo G
Department of Clinical Methodology, 1st Medical School, University of Naples, Italy.
Fetal Diagn Ther. 1991;6(3-4):101-6. doi: 10.1159/000263632.
By using monoclonal antibodies, two-color immunofluorescence techniques and flow cytometry, we evaluated the surface marker phenotypes of lymphocyte subsets in cord blood samples from fetuses in the second trimester of pregnancy. The results indicate that cells of the T-, B- and NK-cell lineages as well as precursor cells can be detected in fetal blood at 18-20 weeks of gestation. At this stage of development, variable proportions of T and B lymphocytes express surface molecules, such as the CD1, CD10, CD38, CD45RA, indicative of a precursor or 'naive' state; on the other hand, the CD57 molecule is not detectable on the membrane of NK and T cells, and the RO isoform of the CD45 leukocyte antigen is synthesized by a low percentage of T cells. We suggest that the observed phenotypic peculiarities of the lymphoid cells might be related to the easy induction of tolerance that occurs in the early ontogenetic stages of the immune system.
通过使用单克隆抗体、双色免疫荧光技术和流式细胞术，我们评估了妊娠中期胎儿脐带血样本中淋巴细胞亚群的表面标志物表型。结果表明，在妊娠18 - 20周时，可在胎儿血液中检测到T细胞、B细胞和NK细胞系以及前体细胞。在这个发育阶段，不同比例的T和B淋巴细胞表达表面分子，如CD1、CD10、CD38、CD45RA，表明处于前体或“幼稚”状态；另一方面，NK和T细胞膜上检测不到CD57分子，且低比例的T细胞合成CD45白细胞抗原的RO同工型。我们认为，观察到的淋巴细胞表型特性可能与免疫系统早期个体发育阶段易于诱导的耐受性有关。
