Zang Hongmin, Xu Dongtan, Chen Ningjie, Li Tao, Li Qingtao, Chen Junchang
Department of Orthopedics, Zibo Central Hospital, Zibo 255036, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2007 Aug;24(4):894-7.
This is an in vitro study to explore the effects of cryopreservation and resuscitation on the biological characteristics of osteoblasts at different times. Osteoblasts taken from the crania of newly born SD rats were cultured. Comparative studies were made on the cells' proliferation, the activity of alkaline phosphatase (ALP), and the number of live cells among fresh cultured osteoblasts and cells after the inception of cryopreservation and resuscitation at time-periods of one, three, six months respectively. The results showed that there were no significant differences among four groups in cell proliferation and in activity of ALP (P > 0.05). Yet, after cryopreservation and resuscitation, there were significant differences between the six-month group and the other three groups (P < 0.05). The results also showed, after cryopreservation and resuscitation, there were no significant differences between the control group and the one-month and three-month groups, respectively (P > 0.05). These findings indicated that the live cells might decrease in number after the osteoblasts were cryopreserved for too long a period, but after cryopreservation and resuscitation, the cells still retained the original biological characteristics of osteoblasts.
这是一项体外研究，旨在探讨不同时间点的冷冻保存和复苏对成骨细胞生物学特性的影响。取新生SD大鼠颅骨的成骨细胞进行培养。分别对新鲜培养的成骨细胞以及冷冻保存和复苏1个月、3个月、6个月后的细胞进行细胞增殖、碱性磷酸酶（ALP）活性及活细胞数量的比较研究。结果显示，四组在细胞增殖和ALP活性方面无显著差异（P>0.05）。然而，冷冻保存和复苏后，6个月组与其他三组之间存在显著差异（P<0.05）。结果还显示，冷冻保存和复苏后，对照组与1个月组和3个月组之间分别无显著差异（P>0.05）。这些研究结果表明，成骨细胞冷冻保存时间过长可能会导致活细胞数量减少，但冷冻保存和复苏后，细胞仍保留成骨细胞原有的生物学特性。
