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三七正丁醇提取物BT-201在体外和胶原诱导的关节炎模型中观察到的抗炎作用。

Anti-inflammatory effects of BT-201, an n-butanol extract of Panax notoginseng, observed in vitro and in a collagen-induced arthritis model.

作者信息

Chang Sun-Hwa, Choi Youngnim, Park Jung-Ae, Jung Dong-Sik, Shin Jieun, Yang Ji-Hyun, Ko Seon-Yle, Kim Se-Won, Kim Jung-Keun

机构信息

Department of Pharmacology and Mechanism, Oscotec Inc., 2-17, Omok-ri, Seonggeo-eup, Cheonan, Choongnam, Republic of Korea.

出版信息

Clin Nutr. 2007 Dec;26(6):785-91. doi: 10.1016/j.clnu.2007.07.008. Epub 2007 Oct 22.

Abstract

BACKGROUND & AIMS: Although there has been some success with protein-based anti-tumor necrosis factor alpha (TNF-alpha) therapeutics, the problems associated with protein-based drugs demand alternative approaches. We screened various herbal extracts for their ability to inhibit TNF-alpha secretions and found that BT-201, an n-butanol extract of Panax notoginseng (Burk.) F. H. Chen (P. notoginseng) has such an ability.

METHODS

The purpose of this study has been to evaluate the anti-inflammatory and anti-rheumatic effects of BT-201. The anti-inflammatory effects were evaluated by measuring the effects of BT-201 on the production of TNF-alpha, interleukin (IL)-1beta, inducible nitric oxide (iNO), and matrix metalloproteinase-13 (MMP-13), in vitro. The anti-rheumatic effects were evaluated by treating mice with collagen-induced arthritis (CIA) using a daily oral administration of BT-201 at 15 mg/kg/day. In addition, the effects on NF-kappaB and mitogen-activated protein kinase (MAPK) pathways were evaluated by Western blotting using phospho-specific antibodies.

RESULTS

BT-201 significantly inhibited all the inflammatory parameters evaluated in vitro and delayed the onset and progression of CIA. BT-201 inhibited the activation of NF-kappaB, ERK, p38, and JNK pathways.

CONCLUSIONS

Our results demonstrated that BT-201 can modulate various aspects of inflammation in vitro and that it has disease-modifying, anti-rheumatic effects in vivo, suggesting that it can be a potential alternative to the current anti-TNF-alpha therapeutics for rheumatoid arthritis and other inflammatory disease.

摘要

背景与目的

尽管基于蛋白质的抗肿瘤坏死因子α(TNF-α)疗法已取得一些成功,但基于蛋白质的药物所带来的问题需要其他替代方法。我们筛选了各种草药提取物抑制TNF-α分泌的能力,发现三七(Panax notoginseng (Burk.) F. H. Chen)正丁醇提取物BT-201具有这种能力。

方法

本研究旨在评估BT-201的抗炎和抗风湿作用。通过体外测量BT-201对TNF-α、白细胞介素(IL)-1β、诱导型一氧化氮(iNO)和基质金属蛋白酶-13(MMP-13)产生的影响来评估其抗炎作用。通过每天以15 mg/kg/天的剂量口服BT-201治疗胶原诱导性关节炎(CIA)小鼠来评估其抗风湿作用。此外,使用磷酸化特异性抗体通过蛋白质印迹法评估对NF-κB和丝裂原活化蛋白激酶(MAPK)途径的影响。

结果

BT-201显著抑制了体外评估的所有炎症参数,并延缓了CIA的发病和进展。BT-201抑制了NF-κB、ERK、p38和JNK途径的激活。

结论

我们的结果表明,BT-201在体外可调节炎症的各个方面,在体内具有改善病情的抗风湿作用,这表明它可能是目前用于类风湿关节炎和其他炎症性疾病的抗TNF-α疗法的潜在替代品。

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