Harada K, Ogawa K, Kimura Y, Murata H, Suzuki M, Thorn P M, Evans W R, Carmichael W W
Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Chem Res Toxicol. 1991 Sep-Oct;4(5):535-40. doi: 10.1021/tx00023a008.
Anabaena flos-aquae NRC 525-17 produces a very potent neurotoxin, anatoxin-a(s). During isolation of the neurotoxin, we found that the strain contains four other toxic compounds which show strong hepatotoxicity. The four toxins, toxins 1, 1', 2, and 3, were successfully purified. Toxin 2, one of major toxins, was identified as 3-desmethylmicrocystin LR (1) by comparison of spectral data of the known compound. Since the three other toxins contain an unknown amino acid, GC/MS was applied and it revealed the presence of homotyrosine in toxins 1 (2) and 1' (3). Only a partial structure was obtained for toxin 3 due to the small amount present in the cells.
水华鱼腥藻NRC 525 - 17能产生一种剧毒神经毒素，即类毒素-a(s)。在分离这种神经毒素的过程中，我们发现该菌株还含有其他四种具有强烈肝毒性的有毒化合物。这四种毒素，毒素1、1'、2和3，已成功纯化。主要毒素之一的毒素2，通过与已知化合物的光谱数据比较，被鉴定为3 - 去甲基微囊藻毒素LR(1)。由于其他三种毒素含有一种未知氨基酸，因此应用气相色谱/质谱联用技术（GC/MS）分析，结果显示毒素1(2)和1'(3)中存在高酪氨酸。由于细胞中毒素3的含量很少，仅获得了其部分结构。
