Simonsen P E, Estambale B B, Agger M
Danish Bilharziasis Laboratory, Charlottenlund, Denmark.
J Helminthol. 1991 Dec;65(4):239-47. doi: 10.1017/s0022149x00010804.
The serum antibody response in golden hamsters (Mesocricetus auratus) infected with the intestinal trematode Echinostoma caproni was examined with ELISA, SDS-PAGE and Western blot, and IFAT techniques. All methods showed that the hamsters responded slowly but developed a clear positive humoral response to the infection. In most hamsters, an antibody response to infection could not be detected earlier than 11-13 weeks after infection with 6 or 25 metacercariae, and responses were weak when compared to previous results from mice infected with the same parasite. IFAT with positive hamster sera on live juvenile E. caproni showed only fluorescence at the posterior tip, which is a different pattern from that seen using from infected mice, indicating a different response to antigens on the juvenile parasites by these two hosts. The results are discussed in relation to the limited selfcure and development of resistance which is observed in golden hamsters infected with E. caproni.
利用酶联免疫吸附测定（ELISA）、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、蛋白质免疫印迹法和间接荧光抗体试验（IFAT）技术，检测了感染肠道吸虫卡氏棘口吸虫的金黄仓鼠（Mesocricetus auratus）的血清抗体反应。所有方法均显示，仓鼠反应缓慢，但对感染产生了明显的阳性体液反应。在大多数仓鼠中，感染6只或25只尾蚴后，在感染后11-13周之前无法检测到对感染的抗体反应，与之前感染相同寄生虫的小鼠的结果相比，反应较弱。用阳性仓鼠血清对活的卡氏棘口吸虫幼虫进行间接荧光抗体试验，仅在虫体后端出现荧光，这与感染小鼠的情况不同，表明这两种宿主对幼虫寄生虫抗原的反应不同。结合在感染卡氏棘口吸虫的金黄仓鼠中观察到的有限自愈和抗性发展情况，对结果进行了讨论。
