Forder R E A, Howarth G S, Tivey D R, Hughes R J
Discipline of Agricultural and Animal Science, School of Agriculture, Food and Wine, University of Adelaide, Roseworthy, 5371, South Australia.
Poult Sci. 2007 Nov;86(11):2396-403. doi: 10.3382/ps.2007-00222.
Mucins possess potential binding sites for both commensal and pathogenic organisms and may perform a defensive role during establishment of the intestinal barrier. To observe the effects of bacteria on intestinal goblet cell mucin production during posthatch development, differences in the small intestine of conventionally reared (CR) and low bacterial load (LBL) broiler chicks were examined. Jejunal and ileal goblet cells were stained with either periodic acid-Schiff stain or high iron diaminealcian blue pH 2.5 to discriminate among neutral, sulfated, and sialylated acidic mucins. Total goblet cell numbers and morphology of goblet cells containing neutral and acidic mucins did not differ significantly between CR and LBL birds. However, significant differences in acidic mucin composition from primarily sulfated to an increase in sialylated sugars at d 4 posthatch were observed in CR chicks, with greater numbers of jejunal and ileal goblet cells displaying this mucin type (CR, 0.5 +/- 0.1 x 10(3) cells/mm(2); LBL, 0.04 +/- 0.02 x10(3) cells/mm(2)). This change in mucin profile in response to bacterial colonization suggests a potential role as a protective mechanism against pathogenic invasion of the intestinal mucosa during early development.
黏蛋白拥有与共生菌和致病菌的潜在结合位点，并且可能在肠道屏障建立过程中发挥防御作用。为了观察细菌对孵化后发育期间肠道杯状细胞黏蛋白产生的影响，研究了常规饲养（CR）和低细菌载量（LBL）肉鸡雏鸡小肠的差异。空肠和回肠杯状细胞用高碘酸-希夫染色或高铁二胺阿尔辛蓝pH 2.5染色，以区分中性、硫酸化和唾液酸化酸性黏蛋白。CR和LBL肉鸡雏鸡之间，总杯状细胞数量以及含有中性和酸性黏蛋白的杯状细胞形态没有显著差异。然而，在CR雏鸡中观察到，孵化后第4天酸性黏蛋白组成从主要硫酸化糖到唾液酸化糖增加有显著差异，空肠和回肠中显示这种黏蛋白类型的杯状细胞数量更多（CR，0.5±0.1×10³个细胞/mm²；LBL，0.04±0.02×10³个细胞/mm²）。这种黏蛋白谱因细菌定植而发生的变化表明，在早期发育过程中，它可能作为一种保护机制，抵御肠道黏膜的病原体入侵。
