Henry S A
J Bacteriol. 1973 Dec;116(3):1293-303. doi: 10.1128/jb.116.3.1293-1303.1973.
Mutants of Saccharomyces cerevisiae having the genotypes fas1 (fatty acid synthetase minus) and fas1, ole1 (fatty acid synthetase and fatty acid desaturase minus) were found to undergo logarithmic death when deprived of required fatty acids, whereas ole1 strains did not. During the first 2 to 3 h of fatty acid starvation, macromolecular synthesis occurred at apparently normal rates, although cell division stopped by the end of the 1st h. Cell death commenced at approximately the 2nd to the 3rd h, and within 24 h, depending upon conditions, 2 to 4 log orders of death had occurred. The loss of viability was accelerated by the addition of detergent, but could be largely prevented by the interruption of protein synthesis, either by amino acid starvation or by the use of cycloheximide. The possible significance of this phenomenon in terms of membrane biosynthesis is discussed.
基因型为fas1（脂肪酸合成酶缺陷型）和fas1、ole1（脂肪酸合成酶和脂肪酸去饱和酶缺陷型）的酿酒酵母突变体在缺乏所需脂肪酸时会经历对数期死亡，而ole1菌株则不会。在脂肪酸饥饿的最初2至3小时内，大分子合成以明显正常的速率进行，尽管细胞分裂在第1小时结束时停止。细胞死亡大约在第2至3小时开始，并且在24小时内，根据条件不同，会发生2至4个对数级别的死亡。添加去污剂会加速活力丧失，但通过氨基酸饥饿或使用环己酰亚胺中断蛋白质合成，可在很大程度上防止这种情况。本文讨论了这一现象在膜生物合成方面可能的意义。
