粘细菌纤维堆囊菌的全基因组序列

Complete genome sequence of the myxobacterium Sorangium cellulosum.

作者信息

Schneiker Susanne, Perlova Olena, Kaiser Olaf, Gerth Klaus, Alici Aysel, Altmeyer Matthias O, Bartels Daniela, Bekel Thomas, Beyer Stefan, Bode Edna, Bode Helge B, Bolten Christoph J, Choudhuri Jomuna V, Doss Sabrina, Elnakady Yasser A, Frank Bettina, Gaigalat Lars, Goesmann Alexander, Groeger Carolin, Gross Frank, Jelsbak Lars, Jelsbak Lotte, Kalinowski Jörn, Kegler Carsten, Knauber Tina, Konietzny Sebastian, Kopp Maren, Krause Lutz, Krug Daniel, Linke Bukhard, Mahmud Taifo, Martinez-Arias Rosa, McHardy Alice C, Merai Michelle, Meyer Folker, Mormann Sascha, Muñoz-Dorado Jose, Perez Juana, Pradella Silke, Rachid Shwan, Raddatz Günter, Rosenau Frank, Rückert Christian, Sasse Florenz, Scharfe Maren, Schuster Stephan C, Suen Garret, Treuner-Lange Anke, Velicer Gregory J, Vorhölter Frank-Jörg, Weissman Kira J, Welch Roy D, Wenzel Silke C, Whitworth David E, Wilhelm Susanne, Wittmann Christoph, Blöcker Helmut, Pühler Alfred, Müller Rolf

机构信息

Department of Genetics, Bielefeld University, PO Box 100131, D-33501 Bielefeld, Germany.

出版信息

Nat Biotechnol. 2007 Nov;25(11):1281-9. doi: 10.1038/nbt1354. Epub 2007 Oct 28.

DOI:10.1038/nbt1354

PMID:17965706

Abstract

The genus Sorangium synthesizes approximately half of the secondary metabolites isolated from myxobacteria, including the anti-cancer metabolite epothilone. We report the complete genome sequence of the model Sorangium strain S. cellulosum So ce56, which produces several natural products and has morphological and physiological properties typical of the genus. The circular genome, comprising 13,033,779 base pairs, is the largest bacterial genome sequenced to date. No global synteny with the genome of Myxococcus xanthus is apparent, revealing an unanticipated level of divergence between these myxobacteria. A large percentage of the genome is devoted to regulation, particularly post-translational phosphorylation, which probably supports the strain's complex, social lifestyle. This regulatory network includes the highest number of eukaryotic protein kinase-like kinases discovered in any organism. Seventeen secondary metabolite loci are encoded in the genome, as well as many enzymes with potential utility in industry.

摘要

堆囊菌属合成了从粘细菌中分离出的约一半次级代谢产物，包括抗癌代谢产物埃坡霉素。我们报告了模式堆囊菌菌株纤维素堆囊菌So ce56的完整基因组序列，该菌株能产生多种天然产物，具有该属典型的形态和生理特性。这个环状基因组由13,033,779个碱基对组成，是迄今为止测序的最大细菌基因组。与黄色粘球菌的基因组没有明显的全局同线性，这揭示了这些粘细菌之间出乎意料的分化程度。基因组的很大一部分用于调控，特别是翻译后磷酸化，这可能支持了该菌株复杂的社会性生活方式。这个调控网络包括在任何生物体中发现的数量最多的真核蛋白激酶样激酶。基因组中编码了17个次级代谢产物位点，以及许多在工业上具有潜在用途的酶。