Kang Chang Duk, Sim Sang Jun
School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
Biotechnol Lett. 2008 Mar;30(3):441-4. doi: 10.1007/s10529-007-9578-0. Epub 2007 Oct 31.
A green, downstream process using common vegetable oils was used for the direct extraction of astaxanthin from Haematococcus. The process consists of a single integrated unit to extract astaxanthin with subsequent separation of the astaxanthin-containing oil extract. Without a cell harvest process step, the culture broth was directly mixed with the vegetable oils; the astaxanthin inside the cell was extracted into the vegetable oil phase by hydrophobic interactions, with recovery yields of 88% and above. The oil extracts were simply separated from the culture medium containing cell debris by gravity settling only.
采用一种绿色的下游工艺，使用常见植物油从雨生红球藻中直接提取虾青素。该工艺由一个单一的集成单元组成，用于提取虾青素，随后分离含虾青素的油提取物。无需细胞收获工艺步骤，将培养液直接与植物油混合；细胞内的虾青素通过疏水相互作用被提取到植物油相中，回收率在88%及以上。仅通过重力沉降就可简单地将油提取物与含有细胞碎片的培养基分离。
