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优化一种嗜盐微藻的类胡萝卜素提取。

Optimization of carotenoid extraction of a halophilic microalgae.

机构信息

Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, College of Life Sciences, Guangxi Normal University, Guilin, China.

Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, Guangxi Normal University, Guilin, China.

出版信息

PLoS One. 2022 Aug 2;17(8):e0270650. doi: 10.1371/journal.pone.0270650. eCollection 2022.

DOI:10.1371/journal.pone.0270650
PMID:35917330
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9345481/
Abstract

Dunaliella parva can produce abundant carotenoids under certain conditions. This paper optimized the extraction efficiency of carotenoids from D. parva. Different organic solvents were examined to determine the most suitable solvent for the extraction. After the determination of the solvent (dimethylsulfoxide, DMSO), the extraction conditions including time, temperature, and volume were then optimized to maximize the extraction efficiency of carotenoids from D. parva using response surface methodology. DMSO was identified as the most suitable solvent. The optimal extraction conditions were as follows: temperature of 57.2°C, time of 11.35 min, the volume of 410 μl, and the optimal extraction efficiency reached 0.517‰. The results showed that the optimal extraction efficiency (0.517‰) improved 31.69% in comparison to the initial extraction efficiency (0.3926‰). In addition, The optimal levels of three influence factors (temperature of 57.2°C, time of 11.35 min, volume of 410 μl) decreased compared with the initial levels (temperature of 60°C, time of 20 min, volume of 1000 μl). In this paper, Central Composite Design (CCD) was used to optimize the extraction efficiency of carotenoids from D. parva, which would lay the groundwork for the extraction and utilization of carotenoids from D. parva in the future.

摘要

小球藻在一定条件下可以产生丰富的类胡萝卜素。本文优化了从小球藻中提取类胡萝卜素的效率。考察了不同的有机溶剂，以确定最适合提取的溶剂。在确定溶剂（二甲基亚砜，DMSO）后，然后使用响应面法优化了时间、温度和体积等提取条件，以最大限度地提高从小球藻中提取类胡萝卜素的效率。DMSO 被确定为最适合的溶剂。最佳提取条件为：温度 57.2°C，时间 11.35 分钟，体积 410μl，最佳提取效率达到 0.517‰。结果表明，与初始提取效率（0.3926‰）相比，最佳提取效率（0.517‰）提高了 31.69%。此外，与初始水平（温度 60°C，时间 20 分钟，体积 1000μl）相比，三个影响因素（温度 57.2°C，时间 11.35 分钟，体积 410μl）的最佳水平有所降低。本文采用中心组合设计（CCD）优化了从小球藻中提取类胡萝卜素的效率，为今后从小球藻中提取和利用类胡萝卜素奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2999/9345481/6f3845242813/pone.0270650.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2999/9345481/16c72b5650c2/pone.0270650.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2999/9345481/de80dce27f9b/pone.0270650.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2999/9345481/6f3845242813/pone.0270650.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2999/9345481/16c72b5650c2/pone.0270650.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2999/9345481/de80dce27f9b/pone.0270650.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2999/9345481/6f3845242813/pone.0270650.g003.jpg

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