Akoua-Koffi C, Akran V, Peenze I, Adjogoua V, de Beer M C, Steele A D, Dosso M, Ehouman A
Département des virus ẃpidémiques, Institut Pasteur de Côte d'Ivoire, BP 490 Abidjan 01, Côte d'Ivoire.
Bull Soc Pathol Exot. 2007 Oct;100(4):246-9.
Viruses, mainly rotaviruses are aetiological agents in more than 80% of the cases of acute diarrhoea in children. In order to determine the epidemiological characteristics and genotypes of human rotaviruses involved in gastroenteritis in diarrheic children aged from 0 to 5 years old in Abidjan, 642 specimens of stools were collected between 1997 and 2000 in the urban health centres and University Teaching Hospitals in Abidjan. The antigenic detection of rotaviruses carried out by ELISA test was followed by the antigenic (VP6 sub-groups) and molecular characterization: polyacrylamide gel electrophoresis and genetic typing. The general prevalence of Rotavirus diarrhoea was 27.9%. Among the children who were found positive, those whose age ranged from 0 to 11 months old accounted for 45.8% against 41.3% and 12.9% for those whose age ranged from 1 to 2 and 3 to 5 years old respectively proving thus the precocity of rotavirus infection. From an electrophoretypical and antigenic point of view 74.5% of 141 extracts of RNA had a "long" profile and belonged to the VP6 II sub-group against 24.8% of "short" profile belonging to sub-group I. The electrophoretypes with short profile were identified in majority in infants whose age ranged from 0 to 2 years old. Out of the P genotypes identified, the P [8] genotype (59.6%) was predominant followed by the P [6] genotype (26.2%), P [4] (2.8%) and one mosaic genotype P[6,8] which represented 11.4%. These results will need to be completed by the determination of VP7 genotypes in order to provide interesting information on rotaviruses before the introduction of anti-Rotavirus vaccines in the country.
病毒，主要是轮状病毒，是80%以上儿童急性腹泻病例的病原体。为了确定阿比让0至5岁腹泻儿童中涉及胃肠炎的人类轮状病毒的流行病学特征和基因型，1997年至2000年期间在阿比让的城市卫生中心和大学教学医院收集了642份粪便样本。通过ELISA试验对轮状病毒进行抗原检测，随后进行抗原（VP6亚组）和分子特征分析：聚丙烯酰胺凝胶电泳和基因分型。轮状病毒腹泻的总体患病率为27.9%。在检测呈阳性的儿童中，年龄在0至11个月的儿童占45.8%，而年龄在1至2岁和3至5岁的儿童分别占41.3%和12.9%，这证明了轮状病毒感染的早熟性。从电泳和抗原角度来看，141份RNA提取物中有74.5%具有“长”型图谱，属于VP6 II亚组，而24.8%的“短”型图谱属于I亚组。短型电泳图谱在0至2岁的婴儿中占多数。在确定的P基因型中，P[8]基因型（59.6%）占主导地位，其次是P[6]基因型（26.2%）、P[4]（2.8%）和一种嵌合基因型P[6,8]，占11.4%。在该国引入抗轮状病毒疫苗之前，需要通过确定VP7基因型来完善这些结果，以便提供有关轮状病毒的有趣信息。
