Agbla Jijoho Mischaël Michel, Capo-Chichi Annick, Agbankpé Alidéhou Jerrold, Dougnon Tamègnon Victorien, Yadouleton Anges William M, Houngbégnon Olivia, Glele-Kakai Clément, Armah George Enyimah, Bankolé Honoré
National Health Laboratory, Ministry of Public Health, 01 P.O. Box, 418 Cotonou, Benin.
Departmental Teaching Hospital Suru-Léré, Pediatric Service, 01 P.O. Box, 1643 Cotonou, Benin.
Int J Microbiol. 2018 May 8;2018:3602967. doi: 10.1155/2018/3602967. eCollection 2018.
Rotavirus remains the main causative agent of gastroenteritis in young children, in countries that have not yet introduced the vaccine. Benin, in order to implement the WHO recommendations, projects to introduce the rotavirus vaccine in 2018 as part of its Expanded Program on Immunization. But before the introduction of this vaccine, epidemiological data on rotavirus infections and rotavirus genotypes circulating in Benin should be available. The aim of this study is to generate epidemiological data on infantile rotavirus diarrhea in Benin. In order to determine the epidemiological characteristics and electrophoretypes of rotavirus responsible for gastroenteritis in diarrheic children aged 0 to 5 years, 186 stool samples were collected according to the WHO Rotavirus Laboratory Manual from March 2014 to February 2015 at Suru-Lere University Hospital Center. Detection of rotavirus antigen was performed by the ELISA test, followed by molecular characterization using polyacrylamide gel electrophoresis. 186 stool samples were analyzed for rotavirus, and seventy-three (39.2%) were found to be positive for rotavirus antigen by ELISA. Children aged 3 to 24 months were the most affected by rotavirus diarrhea in this study. Of the seventy-three children affected with rotavirus diarrhea, 27 (37%) had vomiting accompanied by dehydration and fever. Results based on electrophoresis showed that, among the 73 samples tested, 38 yielded typical rotavirus electrophoretic migration profiles.
在尚未引入疫苗的国家，轮状病毒仍是幼儿肠胃炎的主要病原体。贝宁为落实世界卫生组织的建议，计划于2018年引入轮状病毒疫苗，作为其扩大免疫规划的一部分。但在引入该疫苗之前，应掌握贝宁境内轮状病毒感染情况及流行的轮状病毒基因型的流行病学数据。本研究旨在获取贝宁婴幼儿轮状病毒腹泻的流行病学数据。为确定0至5岁腹泻儿童肠胃炎所涉轮状病毒的流行病学特征及电泳类型，2014年3月至2015年2月期间，按照世界卫生组织《轮状病毒实验室手册》，在苏鲁-莱雷大学医院中心采集了186份粪便样本。通过酶联免疫吸附测定法检测轮状病毒抗原，随后采用聚丙烯酰胺凝胶电泳进行分子特征分析。对186份粪便样本进行轮状病毒分析，通过酶联免疫吸附测定法发现73份（39.2%）轮状病毒抗原呈阳性。本研究中，3至24个月大的儿童受轮状病毒腹泻影响最大。在73名感染轮状病毒腹泻的儿童中，27名（37%）伴有呕吐、脱水和发热症状。电泳结果显示，在检测的73份样本中，38份呈现典型的轮状病毒电泳迁移图谱。
