Bao H-F, Li D, Guo J-H, Lu Z-J, Chen Y-L, Liu Z-X, Liu X-T, Xie Q-G
Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Arch Virol. 2008;153(1):205-9. doi: 10.1007/s00705-007-1082-y. Epub 2007 Nov 8.
Three sets of primers to detect foot-and-mouth disease virus (FMDV) using multiplex RT-PCR were designed based on several reference nucleotide sequences, and their reaction conditions were determined. By testing ten-fold serial dilutions of FMDV, the sensitivity of multiplex RT-PCR is 100 times higher than conventional RT-PCR. Meanwhile, its specificity was confirmed compared with other related vesicular disease viruses. Furthermore, 30 field samples from different animals were tested, and the results supported the method's potential applications in routine veterinary quarantine and epidemic surveillance of FMDV.
基于多个参考核苷酸序列设计了用于通过多重逆转录聚合酶链反应（RT-PCR）检测口蹄疫病毒（FMDV）的三组引物，并确定了它们的反应条件。通过检测FMDV的十倍系列稀释液，多重RT-PCR的灵敏度比传统RT-PCR高100倍。同时，与其他相关水疱性疾病病毒相比，其特异性得到了证实。此外，对来自不同动物的30份现场样本进行了检测，结果支持了该方法在FMDV常规兽医检疫和疫情监测中的潜在应用。
