National Foot-and-Mouth Disease Reference Laboratory of China, Key laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, P.R. China.
Virol J. 2011 Oct 31;8:489. doi: 10.1186/1743-422X-8-489.
A reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay was rapidly used to detect serotype Asia 1 of foot-and-mouth disease virus (FMDV) within 45 min at 61°C. All FMDV serotype Asia 1 reference strains were positive by RT-LAMP, while other viruses such as FMDV serotypes O, C, A and classical swine fever virus, swine vesicular disease virus, porcine reproductive and respiratory syndrome virus and Japanese encephalitis virus remained negative. Furthermore, FMDV sreotype Asia 1 positive samples were able to detect by RT-LAMP assay. This RT-LAMP assay may be suitable particularly for diagnosis of FMDV serotype Asia 1 infection in field stations.
一种逆转录环介导等温扩增（RT-LAMP）检测方法可在 61°C 下 45 分钟内快速检测口蹄疫病毒（FMDV）血清型亚洲 1 型。所有 FMDV 血清型亚洲 1 型参考株经 RT-LAMP 检测均为阳性，而其他病毒如 FMDV 血清型 O、C、A 和经典猪瘟病毒、猪水疱病病毒、猪繁殖与呼吸综合征病毒和日本脑炎病毒则为阴性。此外，RT-LAMP 检测法也可检测到 FMDV 血清型亚洲 1 型阳性样本。这种 RT-LAMP 检测方法特别适用于现场站 FMDV 血清型亚洲 1 型感染的诊断。
