Jarrett E E, Haig D M, McDougall W, McNulty E
Immunology. 1976 May;30(5):671-7.
Primary and booster IgE antibody responses have been elicited in Hooded Lister rats by the intradermal injection or oral administration of very small quantities of egg albumin. Oral immunization was effected by giving antigen by stomach tube or in the drinking water. The minimum primary dose of antigen found to be effective was 1 mug intradermally and 10 mug orally, administered together with an intraperitoneal injection of B. pertussis adjuvant. In rats immunized with these doses secondary responses could be evoked by giving even smaller quantities of antigen, thus 1 ng intradermally or 1 mug orally without adjuvant. Smaller challenge doses were not tried. Large primary doses of antigen (greater than 100 mug) presented by these routes were, on the other hand, found to be inhibitory to the production of secondary IgE responses, this effect being similar to that observed in previously reported intraperitoneal immunization experiments. By contrast with previous experiments, however, tertiary responses could be obtained following immunization by these routes, and we believe this to be reflection of the absorption of smaller and therefore less inhibitory quantities of antigen. Our results are discussed in relation to the control of IgE antibody production, current concepts of the control of antigen absorption through mucosal barriers, and possible implications of the genesis of naturally occurring IgE responses in man.
通过皮内注射或口服极少量卵清蛋白,已在带帽利斯特大鼠中引发了初次和加强型IgE抗体反应。口服免疫通过胃管或饮用水给予抗原实现。发现有效的最小初次抗原剂量为皮内注射1微克和口服10微克,并同时腹腔注射百日咳杆菌佐剂。在用这些剂量免疫的大鼠中,给予甚至更小剂量的抗原即可引发二次反应,因此无佐剂时皮内注射1纳克或口服1微克。未尝试更小的激发剂量。另一方面,通过这些途径给予大剂量的初次抗原(大于100微克)被发现会抑制二次IgE反应的产生,这种效应与先前报道的腹腔免疫实验中观察到的效应相似。然而,与先前的实验相比,通过这些途径免疫后可获得三次反应,我们认为这反映了吸收的抗原量较小,因此抑制作用较小。我们的结果结合IgE抗体产生的控制、当前关于通过黏膜屏障控制抗原吸收的概念以及人类自然发生的IgE反应发生的可能影响进行了讨论。