Dou Jianpeng, Lee Viola S Y, Tzen Jason T C, Lee Maw-Rong
Department of Chemistry, National Chung-Hsing University, Taichung, Taiwan 40227, Republic of China.
Phytochem Anal. 2008 May-Jun;19(3):251-7. doi: 10.1002/pca.1044.
A method was developed to separate and identify acylated flavonol tetraglycosides (AFTGs) by combining isocratic HPLC with electrospray ionisation tandem mass spectrometry. Better separation was obtained for oolong tea infusion using a manually packed Sephadex LH-20 mini-column than with an ACCUBOND ODS solid-phase column. Seven unknown and one known AFTGs were found in oolong teas prepared by various semi-fermentation processes and their structures were identified by mass spectrometry. According to the analyses of diverse oolong teas including Dongding Oolong, Tieguanyin, Wuyi Oolong, Fenghuang Oolong, Gaoshan Shibi, Laocong Shuixian and Baihao Oolong, AFTGs seemed to be universally present, and each oolong tea could be classified into one of three groups (Dongding Oolong, Tieguanyin and Wuyi Oolong) on the basis of its AFTGs profile. The results suggest that the developed method is rapid and sensitive for identifying natural compounds.
通过将等度高效液相色谱法与电喷雾电离串联质谱法相结合，开发了一种分离和鉴定酰化黄酮醇四糖苷（AFTGs）的方法。使用手动填充的葡聚糖LH - 20微型柱对乌龙茶浸出液的分离效果优于ACCUBOND ODS固相柱。在通过各种半发酵工艺制备的乌龙茶中发现了七种未知的和一种已知的AFTGs，并通过质谱法鉴定了它们的结构。根据对包括冻顶乌龙、铁观音、武夷乌龙、凤凰乌龙、高山石笔、老枞水仙和白毫乌龙在内的多种乌龙茶的分析，AFTGs似乎普遍存在，并且每种乌龙茶可以根据其AFTGs谱图分为三组（冻顶乌龙、铁观音和武夷乌龙）之一。结果表明，所开发的方法对于鉴定天然化合物快速且灵敏。
