Ivanov I T, Danailova Iu D
Department of Physics and Biophysics, Medical Institute, Stara Zagora, Bulgaria.
Biofizika. 1991 Sep-Oct;36(5):845-9.
The kinetics of acid hemolysis of human erythrocytes suspended in isotonic sucrose media was investigated. Three successive changes on the curve of the suspension extinction derivative were discriminated and characterized as changes due to morphological transformation, swelling and lysis of the cells. The change due to the reversible morphological transformation could develop in two opposite directions depending on some conditions. The cell swelling was apparently induced by the protonization of hemoglobin with protons entering through the cell membrane. The phases of cell swelling and lysis partly covered each other by isotonicity, but at higher tonicities they were completely separated. These results demonstrate the increased informability of the method applied under such conditions.
研究了悬浮于等渗蔗糖介质中的人红细胞酸溶血动力学。辨别出悬浮液消光导数曲线上的三个连续变化，并将其表征为细胞形态转变、肿胀和裂解引起的变化。由于可逆形态转变引起的变化可能根据某些条件朝两个相反方向发展。细胞肿胀显然是由质子通过细胞膜进入使血红蛋白质子化所诱导的。细胞肿胀和裂解阶段在等渗状态下部分重叠，但在较高渗透压下它们完全分离。这些结果表明在这种条件下应用的方法信息含量增加。
