Hosseini Hamid, Nejabat Mahmood, Mehryar Morsal, Yazdchi Taher, Sedaghat Ahad, Noori Farajollah
Shiraz University of Medical Sciences, Department of Ophthalmology, Khalili hospital, Shiraz, Iran.
Clin Exp Ophthalmol. 2007 Nov;35(8):745-8. doi: 10.1111/j.1442-9071.2007.01572.x.
New and uncontrolled blood vessel development in the cornea is a pivotal process in the pathogenesis of several corneal diseases. These corneal diseases may finally cause blindness and managing them therapeutically is problematic. The data supporting a causal role for vascular endothelial growth factor in corneal neovascularization are extensive. This study aimed to evaluate the effect of subconjunctival bevacizumab (Avastin) on experimental corneal neovascularization in rabbits.
Chemical cauterization of the cornea was performed by touching central cornea with a 5-mm-diameter NaOH-soaked cotton applicator for 10 s in 20 eyes of 20 White New Zealand rabbits. The rabbits were then divided randomly into two equal groups. Bevacizumab (2.5 mg) was administered to 10 eyes (group 1) by a subconjunctival injection immediately after chemical cauterization of corneal surface. As a control, 10 eyes (group 2) received an injection of distilled water. Rabbits were examined daily for detection of the first signs of neovascularization. Three weeks later, the extent of corneal neovascularization was evaluated by direct examination and photograph analyses. Total corneal neovascularization area, degree of circumference involved and longest neovascular pedicle length were assessed.
Bevacizumab significantly decreased the total neovascularization area (P < 0.009), the circumference involved (P < 0.011) and the longest neovascular pedicle length (P < 0.023).
Local injection of bevacizumab has a significant effect on inhibition of alkali burn-induced corneal neovascularization. This shows the potential value of bevacizumab in the treatment of corneal neovascularization.
角膜中新生且不受控制的血管生成是几种角膜疾病发病机制中的关键过程。这些角膜疾病最终可能导致失明，且对其进行治疗存在问题。支持血管内皮生长因子在角膜新生血管形成中起因果作用的数据很广泛。本研究旨在评估结膜下注射贝伐单抗（阿瓦斯汀）对兔实验性角膜新生血管形成的影响。
用直径5毫米的浸有氢氧化钠的棉签接触20只新西兰白兔20只眼的中央角膜10秒，进行角膜化学烧灼。然后将兔子随机分为两组，每组10只眼。角膜表面化学烧灼后立即对10只眼（第1组）进行结膜下注射贝伐单抗（2.5毫克）。作为对照，10只眼（第2组）注射蒸馏水。每天检查兔子以检测新生血管形成的最初迹象。三周后，通过直接检查和照片分析评估角膜新生血管形成的程度。评估角膜新生血管总面积、累及的圆周度和最长新生血管蒂长度。
贝伐单抗显著降低了新生血管总面积（P < 0.009）、累及的圆周度（P < 0.011）和最长新生血管蒂长度（P < 0.023）。
局部注射贝伐单抗对抑制碱烧伤诱导的角膜新生血管形成有显著作用。这表明贝伐单抗在治疗角膜新生血管形成方面具有潜在价值。
