Vandevoort Catherine A, Thirkill Twanda L, Douglas Gordon C
California National Primate Research Center, Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis CA 95616, USA.
Stem Cells Dev. 2007 Oct;16(5):779-88. doi: 10.1089/scd.2007.0020.
Although trophoblast stem cells can be obtained directly from blastocyst outgrowths in the mouse, this has never been described in primates. In human and non-human primates, trophoblast cells have been obtained from embryonic stem (ES) cells or embryoid bodies (EBs). The results reported here show for the first time that cells with the characteristics of trophoblast stem cells can be derived directly from rhesus monkey blastocyst outgrowths. The cells expressed trophoblast markers and were maintained for multiple passages in the absence of feeder layers or growth factors. The cells could be maintained as adherent, mononuclear cells by regular passaging, but they formed syncytial-like structures if maintained in culture for prolonged periods or if incubated in the presence of 17beta-estradiol. The cells also demonstrated invasive behavior similar to extravillous trophoblasts. The availability of these lines provides a useful experimental system for studying trophoblast differentiation and for developing novel intervention strategies to treat placental dysfunction.
尽管滋养层干细胞可直接从小鼠囊胚的体外生长物中获取，但在灵长类动物中从未有过此类报道。在人类和非人类灵长类动物中，滋养层细胞是从胚胎干细胞（ES）或胚状体（EB）中获得的。此处报道的结果首次表明，具有滋养层干细胞特征的细胞可直接从恒河猴囊胚的体外生长物中获得。这些细胞表达滋养层标记物，并且在没有饲养层或生长因子的情况下可传代培养多次。通过定期传代，这些细胞可以作为贴壁单核细胞维持，但如果长时间培养或在17β-雌二醇存在下孵育，它们会形成合体样结构。这些细胞还表现出与绒毛外滋养层细胞相似的侵袭行为。这些细胞系的获得为研究滋养层分化以及开发治疗胎盘功能障碍的新型干预策略提供了一个有用的实验系统。
